Separation and Analysis of Adherent and Non-Adherent Cancer Cells Using a Single-Cell Microarray Chip
AbstractA new single-cell microarray chip was designed and developed to separate and analyze single adherent and non-adherent cancer cells. The single-cell microarray chip is made of polystyrene with over 60,000 microchambers of 10 different size patterns (31–40 µm upper diameter, 11–20 µm lower diameter). A drop of suspension of adherent carcinoma (NCI-H1650) and non-adherent leukocyte (CCRF-CEM) cells was placed onto the chip, and single-cell occupancy of NCI-H1650 and CCRF-CEM was determined to be 79% and 84%, respectively. This was achieved by controlling the chip design and surface treatment. Analysis of protein expression in single NCI-H1650 and CCRF-CEM cells was performed on the single-cell microarray chip by multi-antibody staining. Additionally, with this system, we retrieved positive single cells from the microchambers by a micromanipulator. Thus, this system demonstrates the potential for easy and accurate separation and analysis of various types of single cells. View Full-Text
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Yamamura, S.; Yamada, E.; Kimura, F.; Miyajima, K.; Shigeto, H. Separation and Analysis of Adherent and Non-Adherent Cancer Cells Using a Single-Cell Microarray Chip. Sensors 2017, 17, 2410.
Yamamura S, Yamada E, Kimura F, Miyajima K, Shigeto H. Separation and Analysis of Adherent and Non-Adherent Cancer Cells Using a Single-Cell Microarray Chip. Sensors. 2017; 17(10):2410.Chicago/Turabian Style
Yamamura, Shohei; Yamada, Eriko; Kimura, Fukiko; Miyajima, Kumiko; Shigeto, Hajime. 2017. "Separation and Analysis of Adherent and Non-Adherent Cancer Cells Using a Single-Cell Microarray Chip." Sensors 17, no. 10: 2410.
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