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Sensors 2017, 17(10), 2271; https://doi.org/10.3390/s17102271

Reconfigurable Microfluidic Magnetic Valve Arrays: Towards a Radiotherapy-Compatible Spheroid Culture Platform for the Combinatorial Screening of Cancer Therapies

1
Biomedical Engineering Institute, École Polytechnique de Montréal, Montreal, H3T 1J4, Canada
2
Institut du Cancer de Montréal, Université de Montréal, Montréal, H3T 1J4, Canada
3
Centre de Recherche du Centre Hospitalier de l’Université de Montréal, H2X 0A9, Montréal, Canada
4
Department of Engineering Physics, École Polytechnique de Montréal, Montreal, H3T 1J4, Canada
5
Department of Radiation Oncology, Centre Hospitalier de l’Université de Montréal, Montréal, H2X 0A9, Canada
*
Author to whom correspondence should be addressed.
Received: 29 August 2017 / Revised: 26 September 2017 / Accepted: 27 September 2017 / Published: 4 October 2017
(This article belongs to the Special Issue Microfluidics-Based Microsystem Integration Research)
View Full-Text   |   Download PDF [2334 KB, uploaded 10 October 2017]   |  

Abstract

We introduce here a microfluidic cell culture platform or spheroid culture chamber array (SCCA) that can synthesize, culture, and enable fluorescence imaging of 3D cell aggregates (typically spheroids) directly on-chip while specifying the flow of reagents in each chamber via the use of an array of passive magnetic valves. The SCCA valves demonstrated sufficient resistance to burst (above 100 mBar), including after receiving radiotherapy (RT) doses of up to 8 Gy combined with standard 37 °C incubation for up to 7 days, enabling the simultaneous synthesis of multiple spheroids from different cell lines on the same array. Our results suggest that SCCA would be an asset in drug discovery processes, seeking to identify combinatorial treatments. View Full-Text
Keywords: microfluidics; radiotherapy; chemotherapy; spheroids; drug discovery; valves microfluidics; radiotherapy; chemotherapy; spheroids; drug discovery; valves
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Brunet, A.R.; Labelle, F.; Wong, P.; Gervais, T. Reconfigurable Microfluidic Magnetic Valve Arrays: Towards a Radiotherapy-Compatible Spheroid Culture Platform for the Combinatorial Screening of Cancer Therapies. Sensors 2017, 17, 2271.

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