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Sensors 2017, 17(10), 2188;

Raman Hyperspectral Imaging for Detection of Watermelon Seeds Infected with Acidovorax citrulli

Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Powder Mill Rd. Bldg. 303, BARC-East, Beltsville, MD 20705, USA
Department of Biosystems Machinery Engineering, College of Agricultural and Life Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea
Department of Horticultural Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 441-701, Korea
Author to whom correspondence should be addressed.
Received: 19 July 2017 / Revised: 22 August 2017 / Accepted: 18 September 2017 / Published: 23 September 2017
(This article belongs to the Section Biosensors)
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The bacterial infection of seeds is one of the most important quality factors affecting yield. Conventional detection methods for bacteria-infected seeds, such as biological, serological, and molecular tests, are not feasible since they require expensive equipment, and furthermore, the testing processes are also time-consuming. In this study, we use the Raman hyperspectral imaging technique to distinguish bacteria-infected seeds from healthy seeds as a rapid, accurate, and non-destructive detection tool. We utilize Raman hyperspectral imaging data in the spectral range of 400–1800 cm−1 to determine the optimal band-ratio for the discrimination of watermelon seeds infected by the bacteria Acidovorax citrulli using ANOVA. Two bands at 1076.8 cm−1 and 437 cm−1 are selected as the optimal Raman peaks for the detection of bacteria-infected seeds. The results demonstrate that the Raman hyperspectral imaging technique has a good potential for the detection of bacteria-infected watermelon seeds and that it could form a suitable alternative to conventional methods. View Full-Text
Keywords: Raman hyperspectral imaging; spectral analysis; image processing; seed quality Raman hyperspectral imaging; spectral analysis; image processing; seed quality

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Lee, H.; Kim, M.S.; Qin, J.; Park, E.; Song, Y.-R.; Oh, C.-S.; Cho, B.-K. Raman Hyperspectral Imaging for Detection of Watermelon Seeds Infected with Acidovorax citrulli. Sensors 2017, 17, 2188.

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