Next Article in Journal
Ambient Sound-Based Collaborative Localization of Indeterministic Devices
Next Article in Special Issue
Biomimetic Precapillary Flow Patterns for Enhancing Blood Plasma Separation: A Preliminary Study
Previous Article in Journal
Modeling and Analysis of a 2-DOF Spherical Parallel Manipulator
Previous Article in Special Issue
A Microfluidic Approach for Inducing Cell Rotation by Means of Hydrodynamic Forces
Article Menu

Export Article

Open AccessArticle
Sensors 2016, 16(9), 1489; doi:10.3390/s16091489

A Microchip for Integrated Single-Cell Gene Expression Profiling and Genotoxicity Detection

School of Mechanical Engineering and Automation, Fuzhou University, Fujian 350116, China
*
Author to whom correspondence should be addressed.
Academic Editors: Fan-Gang Tseng and Tuhin Subhra Santra
Received: 16 July 2016 / Revised: 28 August 2016 / Accepted: 6 September 2016 / Published: 14 September 2016
(This article belongs to the Special Issue Biomicrofluidics)
View Full-Text   |   Download PDF [2961 KB, uploaded 14 September 2016]   |  

Abstract

Microfluidics-based single-cell study is an emerging approach in personalized treatment or precision medicine studies. Single-cell gene expression holds a potential to provide treatment selections with maximized efficacy to help cancer patients based on a genetic understanding of their disease. This work presents a multi-layer microchip for single-cell multiplexed gene expression profiling and genotoxicity detection. Treated by three drug reagents (i.e., methyl methanesulfonate, docetaxel and colchicine) with varied concentrations and time lengths, individual human cancer cells (MDA-MB-231) are lysed on-chip, and the released mRNA templates are captured and reversely transcribed into single strand DNA. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), cyclin-dependent kinase inhibitor 1A (CDKN1A), and aurora kinase A (AURKA) genes from single cells are amplified and real-time quantified through multiplex polymerase chain reaction. The microchip is capable of integrating all steps of single-cell multiplexed gene expression profiling, and providing precision detection of drug induced genotoxic stress. Throughput has been set to be 18, and can be further increased following the same approach. Numerical simulation of on-chip single cell trapping and heat transfer has been employed to evaluate the chip design and operation. View Full-Text
Keywords: single-cell analysis; integrated multiplex RT-qPCR; microfluidics single-cell analysis; integrated multiplex RT-qPCR; microfluidics
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Dong, H.; Sun, H. A Microchip for Integrated Single-Cell Gene Expression Profiling and Genotoxicity Detection. Sensors 2016, 16, 1489.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top