A Simple BODIPY-Based Viscosity Probe for Imaging of Cellular Viscosity in Live Cells
AbstractIntracellular viscosity is a fundamental physical parameter that indicates the functioning of cells. In this work, we developed a simple boron-dipyrromethene (BODIPY)-based probe, BTV, for cellular mitochondria viscosity imaging by coupling a simple BODIPY rotor with a mitochondria-targeting unit. The BTV exhibited a significant fluorescence intensity enhancement of more than 100-fold as the solvent viscosity increased. Also, the probe showed a direct linear relationship between the fluorescence lifetime and the media viscosity, which makes it possible to trace the change of the medium viscosity. Furthermore, it was demonstrated that BTV could achieve practical applicability in the monitoring of mitochondrial viscosity changes in live cells through fluorescence lifetime imaging microscopy (FLIM). View Full-Text
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Su, D.; Teoh, C.L.; Gao, N.; Xu, Q.-H.; Chang, Y.-T. A Simple BODIPY-Based Viscosity Probe for Imaging of Cellular Viscosity in Live Cells. Sensors 2016, 16, 1397.
Su D, Teoh CL, Gao N, Xu Q-H, Chang Y-T. A Simple BODIPY-Based Viscosity Probe for Imaging of Cellular Viscosity in Live Cells. Sensors. 2016; 16(9):1397.Chicago/Turabian Style
Su, Dongdong; Teoh, Chai L.; Gao, Nengyue; Xu, Qing-Hua; Chang, Young-Tae. 2016. "A Simple BODIPY-Based Viscosity Probe for Imaging of Cellular Viscosity in Live Cells." Sensors 16, no. 9: 1397.
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