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Sensors 2016, 16(9), 1397; doi:10.3390/s16091397

A Simple BODIPY-Based Viscosity Probe for Imaging of Cellular Viscosity in Live Cells

1
Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore 138667, Singapore
2
Department of Chemistry, National University of Singapore, Singapore 117543, Singapore
*
Author to whom correspondence should be addressed.
Academic Editors: Jong Seung Kim and Min Hee Lee
Received: 2 August 2016 / Revised: 23 August 2016 / Accepted: 26 August 2016 / Published: 31 August 2016
(This article belongs to the Special Issue Colorimetric and Fluorescent Sensor)
View Full-Text   |   Download PDF [2423 KB, uploaded 31 August 2016]   |  

Abstract

Intracellular viscosity is a fundamental physical parameter that indicates the functioning of cells. In this work, we developed a simple boron-dipyrromethene (BODIPY)-based probe, BTV, for cellular mitochondria viscosity imaging by coupling a simple BODIPY rotor with a mitochondria-targeting unit. The BTV exhibited a significant fluorescence intensity enhancement of more than 100-fold as the solvent viscosity increased. Also, the probe showed a direct linear relationship between the fluorescence lifetime and the media viscosity, which makes it possible to trace the change of the medium viscosity. Furthermore, it was demonstrated that BTV could achieve practical applicability in the monitoring of mitochondrial viscosity changes in live cells through fluorescence lifetime imaging microscopy (FLIM). View Full-Text
Keywords: BODIPY; fluorescent probes; sensors; viscosity; FLIM; cell imaging BODIPY; fluorescent probes; sensors; viscosity; FLIM; cell imaging
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MDPI and ACS Style

Su, D.; Teoh, C.L.; Gao, N.; Xu, Q.-H.; Chang, Y.-T. A Simple BODIPY-Based Viscosity Probe for Imaging of Cellular Viscosity in Live Cells. Sensors 2016, 16, 1397.

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