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Sensors 2016, 16(5), 701; doi:10.3390/s16050701

Influences of Probe’s Morphology for Metal Ion Detection Based on Light-Addressable Potentiometric Sensors

1,†
,
2,†
,
3
,
4
and
1,*
1
College of Information Science Technology, Nankai University, No. 94 Weijin Road, Nankai District, Tianjin 300071, China
2
Department of Immunology, School of Medicine, Nankai University, No. 94 Weijin Road, Nankai District, Tianjin 300071, China
3
College of Chemistry, Nankai University, No. 94 Weijin Road, Nankai District, Tianjin 300071, China
4
School of Medical Laboratory, Tianjin Medical University, No. 1 Guangdong Road, Hexi District, Tianjin 300203, China
Co-First Author.
*
Author to whom correspondence should be addressed.
Academic Editor: W. Rudolf Seitz
Received: 22 February 2016 / Revised: 22 April 2016 / Accepted: 27 April 2016 / Published: 14 May 2016
(This article belongs to the Section Chemical Sensors)
View Full-Text   |   Download PDF [2297 KB, uploaded 14 May 2016]   |  

Abstract

The sensing mechanism of binding Hg2+ into thymine-thymine (T-T) mismatched base pairs was introduced into a light-addressable potentiometric sensor (LAPS) with anti-Hg2+ aptamer as the sensing units. Three kinds of T-rich single-strand DNA (ssDNA) chains with different spacer lengths, from 0 to 12 –CH2 groups, were designed to investigate surface charge and morphological effects on the LAPS’ output. First, by comparing the responding of LAPS modified with three kinds of ssDNA, it was found that the best performance for Hg2+ sensing was exhibited by the probe without –CH2 groups. The detection limit of Hg2+ ion was 1 ppt under the optimal condition. Second, the cooperative effects of surface charge and morphology on the output were observed by the controlled experiments. The two effects were the negative charge balanced by metal cations and the morphological changing caused by the formation of T-Hg2+-T structure. In conclusion, not only the influences of the aptamer probe’s morphology and surface charge was investigated on the platform of LAPS, but also sensing Hg2+ ions was achieved for the first time by the presented aptamer LAPS. View Full-Text
Keywords: light addressable potentiometric sensor (LAPS); ssDNA; aptamer; morphology; metal ion light addressable potentiometric sensor (LAPS); ssDNA; aptamer; morphology; metal ion
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Shao, C.; Zhou, S.; Yin, X.; Gu, Y.; Jia, Y. Influences of Probe’s Morphology for Metal Ion Detection Based on Light-Addressable Potentiometric Sensors. Sensors 2016, 16, 701.

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