Nitrogen-Doped Carbon Dots as A New Substrate for Sensitive Glucose Determination
AbstractNitrogen-doped carbon dots are introduced as a novel substrate suitable for enzyme immobilization in electrochemical detection metods. Nitrogen-doped carbon dots are easily synthesised from polyacrylamide in just one step. With the help of the amino group on chitosan, glucose oxidase is immobilized on nitrogen-doped carbon dots-modified carbon glassy electrodes by amino-carboxyl reactions. The nitrogen-induced charge delocalization at nitrogen-doped carbon dots can enhance the electrocatalytic activity toward the reduction of O2. The specific amino-carboxyl reaction provides strong and stable immobilization of GOx on electrodes. The developed biosensor responds efficiently to the presence of glucose in serum samples over the concentration range from 1 to 12 mM with a detection limit of 0.25 mM. This novel biosensor has good reproducibility and stability, and is highly selective for glucose determination under physiological conditions. These results indicate that N-doped quantum dots represent a novel candidate material for the construction of electrochemical biosensors. View Full-Text
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Ji, H.; Zhou, F.; Gu, J.; Shu, C.; Xi, K.; Jia, X. Nitrogen-Doped Carbon Dots as A New Substrate for Sensitive Glucose Determination. Sensors 2016, 16, 630.
Ji H, Zhou F, Gu J, Shu C, Xi K, Jia X. Nitrogen-Doped Carbon Dots as A New Substrate for Sensitive Glucose Determination. Sensors. 2016; 16(5):630.Chicago/Turabian Style
Ji, Hanxu; Zhou, Feng; Gu, Jiangjiang; Shu, Chen; Xi, Kai; Jia, Xudong. 2016. "Nitrogen-Doped Carbon Dots as A New Substrate for Sensitive Glucose Determination." Sensors 16, no. 5: 630.
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