Next Article in Journal
Localized Electrical Impedance Myography of the Biceps Brachii Muscle during Different Levels of Isometric Contraction and Fatigue
Next Article in Special Issue
A Rapid In Situ Colorimetric Assay for Cobalt Detection by the Naked Eye
Previous Article in Journal
Vision-Based Leader Vehicle Trajectory Tracking for Multiple Agricultural Vehicles
Previous Article in Special Issue
Rapid Detection of Glycogen Synthase Kinase-3 Activity in Mouse Sperm Using Fluorescent Gel Shift Electrophoresis
Article Menu

Export Article

Open AccessArticle
Sensors 2016, 16(4), 584; doi:10.3390/s16040584

Optimizing Colorimetric Assay Based on V2O5 Nanozymes for Sensitive Detection of H2O2 and Glucose

School of Public Health, Jilin University, Changchun 130021, China
*
Author to whom correspondence should be addressed.
Academic Editors: Jong Seung Kim and Min Hee Lee
Received: 1 March 2016 / Revised: 11 April 2016 / Accepted: 19 April 2016 / Published: 22 April 2016
(This article belongs to the Special Issue Colorimetric and Fluorescent Sensor)
View Full-Text   |   Download PDF [2069 KB, uploaded 22 April 2016]   |  

Abstract

Nanozyme-based chemical sensing is a rapidly emerging field of research. Herein, a simple colorimetric assay for the detection of hydrogen peroxide and glucose based on the peroxidase-like activity of V2O5 nanozymes has been established. In this assay, the effects of pH, substrate, nanozyme concentrations and buffer solution have been investigated. It was found that compared with 3,3′,5,5′-tetramethylbenzidine (TMB), the enzyme substrate o-phenylenediamine (OPD) seriously interfered with the H2O2 detection. Under the optimal reaction conditions, the resulting sensor displayed a good response to H2O2 with a linear range of 1 to 500 μM, and a detection limit of 1 μM at a signal-to-noise ratio of 3. A linear correlation was established between absorbance intensity and concentration of glucose from 10 to 2000 μM, with a detection limit of 10 μM. The current work presents a simple, cheap, more convenient, sensitive, and easy handling colorimetric assay. View Full-Text
Keywords: V2O5 nanozymes; variation; substrates; H2O2; glucose V2O5 nanozymes; variation; substrates; H2O2; glucose
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Sun, J.; Li, C.; Qi, Y.; Guo, S.; Liang, X. Optimizing Colorimetric Assay Based on V2O5 Nanozymes for Sensitive Detection of H2O2 and Glucose. Sensors 2016, 16, 584.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top