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Sensors 2016, 16(3), 308; doi:10.3390/s16030308

An Electrochemiluminescence Immunosensor Based on Gold-Magnetic Nanoparticles and Phage Displayed Antibodies

State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China
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Author to whom correspondence should be addressed.
Academic Editor: Huangxian Ju
Received: 30 November 2015 / Revised: 28 January 2016 / Accepted: 28 January 2016 / Published: 27 February 2016
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [3388 KB, uploaded 27 February 2016]   |  

Abstract

Using the multiple advantages of the ultra-highly sensitive electrochemiluminescence (ECL) technique, Staphylococcus protein A (SPA) functionalized gold-magnetic nanoparticles and phage displayed antibodies, and using gold-magnetic nanoparticles coated with SPA and coupled with a polyclonal antibody (pcAb) as magnetic capturing probes, and Ru(bpy)32+-labeled phage displayed antibody as a specific luminescence probe, this study reports a new way to detect ricin with a highly sensitive and specific ECL immunosensor and amplify specific detection signals. The linear detection range of the sensor was 0.0001~200 µg/L, and the limit of detection (LOD) was 0.0001 µg/L, which is 2500-fold lower than that of the conventional ELISA technique. The gold-magnetic nanoparticles, SPA and Ru(bpy)32+-labeled phage displayed antibody displayed different amplifying effects in the ECL immunosensor and can decrease LOD 3-fold, 3-fold and 20-fold, respectively, compared with the ECL immunosensors without one of the three effects. The integrated amplifying effect can decrease the LOD 180-fold. The immunosensor integrates the unique advantages of SPA-coated gold-magnetic nanoparticles that improve the activity of the functionalized capturing probe, and the amplifying effect of the Ru(bpy)32+-labeled phage displayed antibodies, so it increases specificity, interference-resistance and decreases LOD. It is proven to be well suited for the analysis of trace amounts of ricin in various environmental samples with high recovery ratios and reproducibility. View Full-Text
Keywords: gold-magnetic nanoparticles; phage displayed antibody; Staphylococcus protein A; ECL immunosensor; ricin gold-magnetic nanoparticles; phage displayed antibody; Staphylococcus protein A; ECL immunosensor; ricin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Mu, X.; Tong, Z.; Huang, Q.; Liu, B.; Liu, Z.; Hao, L.; Dong, H.; Zhang, J.; Gao, C. An Electrochemiluminescence Immunosensor Based on Gold-Magnetic Nanoparticles and Phage Displayed Antibodies. Sensors 2016, 16, 308.

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