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Sensors 2016, 16(2), 188; doi:10.3390/s16020188

An Electrochemical Impedance Spectroscopy System for Monitoring Pineapple Waste Saccharification

Instituto de Ingeniería de Alimentos para el Desarrollo (IIAD), Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain
Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universitat Politècnica de València—Universitat de València, Camí de Vera s/n, 46022 Valencia, Spain
Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 15 December 2015 / Revised: 27 January 2016 / Accepted: 31 January 2016 / Published: 4 February 2016
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [2706 KB, uploaded 4 February 2016]   |  


Electrochemical impedance spectroscopy (EIS) has been used for monitoring the enzymatic pineapple waste hydrolysis process. The system employed consists of a device called Advanced Voltammetry, Impedance Spectroscopy & Potentiometry Analyzer (AVISPA) equipped with a specific software application and a stainless steel double needle electrode. EIS measurements were conducted at different saccharification time intervals: 0, 0.75, 1.5, 6, 12 and 24 h. Partial least squares (PLS) were used to model the relationship between the EIS measurements and the sugar determination by HPAEC-PAD. On the other hand, artificial neural networks: (multilayer feed forward architecture with quick propagation training algorithm and logistic-type transfer functions) gave the best results as predictive models for glucose, fructose, sucrose and total sugars. Coefficients of determination (R2) and root mean square errors of prediction (RMSEP) were determined as R2 > 0.944 and RMSEP < 1.782 for PLS and R2 > 0.973 and RMSEP < 0.486 for artificial neural networks (ANNs), respectively. Therefore, a combination of both an EIS-based technique and ANN models is suggested as a promising alternative to the traditional laboratory techniques for monitoring the pineapple waste saccharification step. View Full-Text
Keywords: electrochemical impedance spectroscopy; saccharification; monitoring; pineapple waste electrochemical impedance spectroscopy; saccharification; monitoring; pineapple waste

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Conesa, C.; Ibáñez Civera, J.; Seguí, L.; Fito, P.; Laguarda-Miró, N. An Electrochemical Impedance Spectroscopy System for Monitoring Pineapple Waste Saccharification. Sensors 2016, 16, 188.

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