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Sensors 2016, 16(12), 2174; doi:10.3390/s16122174

Development and Application of a Synthetically-Derived Lead Biosensor Construct for Use in Gram-Negative Bacteria

Applied and Environmental Microbiology Laboratory, Department of Physiology, Anatomy and Microbiology, La Trobe University, Melbourne, Victoria 3086, Australia
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Author to whom correspondence should be addressed.
Academic Editor: Russell Binions
Received: 10 November 2016 / Revised: 9 December 2016 / Accepted: 13 December 2016 / Published: 18 December 2016
(This article belongs to the Special Issue Sensors for Environmental Monitoring 2016)
View Full-Text   |   Download PDF [2213 KB, uploaded 20 December 2016]   |  

Abstract

The use of lead in manufacturing has decreased significantly over the last few decades. However, previous widespread use of lead-containing products and their incorrect disposal has resulted in environmental contamination. Accumulation of harmful quantities of lead pose a threat to all living organisms, through inhalation, ingestion, or direct contact, resulting in lead poisoning. This study utilized synthetic biology principles to develop plasmid-based whole-cell bacterial biosensors for detection of lead. The genetic element of the lead biosensor construct consists of pbrR, which encodes the regulatory protein, together with its divergent promoter region and a promoterless gfp. GFP expression is controlled by PbrR in response to the presence of lead. The lead biosensor genetic element was cloned onto a low-copy number broad host range plasmid, which can stably exist in a range of laboratory and environmental isolates, including Pseudomonas, Shewanella, and Enterobacter. The biosensors constructed were found to be sensitive, rapid, and specific and could, as such, serve as monitoring tools for lead-contaminated water. View Full-Text
Keywords: whole cell biosensors; lead; synthetic biology; environmental monitoring whole cell biosensors; lead; synthetic biology; environmental monitoring
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Bereza-Malcolm, L.; Aracic, S.; Franks, A.E. Development and Application of a Synthetically-Derived Lead Biosensor Construct for Use in Gram-Negative Bacteria. Sensors 2016, 16, 2174.

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