Next Article in Journal
Rapid Detection Methods for Asphalt Pavement Thicknesses and Defects by a Vehicle-Mounted Ground Penetrating Radar (GPR) System
Next Article in Special Issue
Domain 4 (D4) of Perfringolysin O to Visualize Cholesterol in Cellular Membranes—The Update
Previous Article in Journal
Detection of Ciprofloxacin in Urine through Sensitized Lanthanide Luminescence
Article Menu

Export Article

Open AccessArticle
Sensors 2016, 16(12), 2068; doi:10.3390/s16122068

Electrical Impedance Monitoring of C2C12 Myoblast Differentiation on an Indium Tin Oxide Electrode

1
Department of Biomedical Engineering, Gachon University, Incheon 21936, Korea
2
College of Pharmacy and Gachon Institute of Pharmaceutical Science, Gachon University, Incheon 21936, Korea
3
Lee Gil Ya Cancer and Diabetes Institute, Incheon 21999, Korea
4
Department of Materials Science and Engineering, Yonsei University, Seodaemun-gu, Seoul 120-749, Korea
5
Gachon Advanced Institute for Health Science & Technology, Gachon University, Incheon 21999, Korea
6
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 04107, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Yong Liu
Received: 31 October 2016 / Revised: 29 November 2016 / Accepted: 30 November 2016 / Published: 5 December 2016
(This article belongs to the Special Issue Whole Cell-Based Biosensors and Application)
View Full-Text   |   Download PDF [3560 KB, uploaded 5 December 2016]   |  

Abstract

Electrical cell-substrate impedance sensing is increasingly being used for label-free and real-time monitoring of changes in cell morphology and number during cell growth, drug screening, and differentiation. In this study, we evaluated the feasibility of using ECIS to monitor C2C12 myoblast differentiation using a fabricated indium tin oxide (ITO) electrode-based chip. C2C12 myoblast differentiation on the ITO electrode was validated based on decreases in the mRNA level of MyoD and increases in the mRNA levels of myogenin and myosin heavy chain (MHC). Additionally, MHC expression and morphological changes in myoblasts differentiated on the ITO electrode were comparable to those in cells in the control culture dish. From the monitoring the integration of the resistance change at 21.5 kHz, the cell differentiation was label-free and real-time detectable in 30 h of differentiation (p < 0.05). View Full-Text
Keywords: electric cell-substrate impedance sensing; label-free; real-time; C2C12 cells; myoblast differentiation electric cell-substrate impedance sensing; label-free; real-time; C2C12 cells; myoblast differentiation
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Park, I.; Hong, Y.; Jun, Y.-H.; Lee, G.-Y.; Jun, H.-S.; Pyun, J.-C.; Choi, J.-W.; Cho, S. Electrical Impedance Monitoring of C2C12 Myoblast Differentiation on an Indium Tin Oxide Electrode. Sensors 2016, 16, 2068.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top