Next Article in Journal
Wireless Mid-Infrared Spectroscopy Sensor Network for Automatic Carbon Dioxide Fertilization in a Greenhouse Environment
Next Article in Special Issue
Comparing ∆Tmax Determination Approaches for Granier-Based Sapflow Estimations
Previous Article in Journal
Virtual Distances Methodology as Verification Technique for AACMMs with a Capacitive Sensor Based Indexed Metrology Platform
Previous Article in Special Issue
In-Field, In Situ, and In Vivo 3-Dimensional Elemental Mapping for Plant Tissue and Soil Analysis Using Laser-Induced Breakdown Spectroscopy
Article Menu

Export Article

Open AccessArticle
Sensors 2016, 16(11), 1945; doi:10.3390/s16111945

Quencher-Free Fluorescence Method for the Detection of Mercury(II) Based on Polymerase-Aided Photoinduced Electron Transfer Strategy

1
State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410013, China
2
Department of Environmental Monitoring, Changsha Environmental Protection Technology Vocational College, Changsha 410004, China
3
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410081, China
*
Author to whom correspondence should be addressed.
Academic Editor: Russell Binions
Received: 16 October 2016 / Revised: 10 November 2016 / Accepted: 15 November 2016 / Published: 18 November 2016
(This article belongs to the Special Issue Sensors for Environmental Monitoring 2016)
View Full-Text   |   Download PDF [1209 KB, uploaded 18 November 2016]   |  

Abstract

A new quencher-free Hg2+ ion assay method was developed based on polymerase-assisted photoinduced electron transfer (PIET). In this approach, a probe is designed with a mercury ion recognition sequence (MRS) that is composed of two T-rich functional areas separated by a spacer of random bases at the 3′-end, and a sequence of stacked cytosines at the 5′-end, to which a fluorescein (FAM) is attached. Upon addition of Hg2+ ions into this sensing system, the MRS folds into a hairpin structure at the 3′-end with Hg2+-mediated base pairs. In the presence of DNA polymerase, it will catalyze the extension reaction, resulting in the formation of stacked guanines, which will instantly quench the fluorescence of FAM through PIET. Under optimal conditions, the limit of detection for Hg2+ ions was estimated to be 5 nM which is higher than the US Environmental Protection Agency (EPA) standard limit. In addition, no labeling with a quencher was requiring, and the present method is fairly simple, fast and low cost. It is expected that this cost-effective fluorescence method might hold considerable potential in the detection of Hg2+ ions in real biological and environmental samples. View Full-Text
Keywords: quencher-free; Hg2+ ion; photoinduced electron transfer quencher-free; Hg2+ ion; photoinduced electron transfer
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Liu, H.; Ma, L.; Ma, C.; Du, J.; Wang, M.; Wang, K. Quencher-Free Fluorescence Method for the Detection of Mercury(II) Based on Polymerase-Aided Photoinduced Electron Transfer Strategy. Sensors 2016, 16, 1945.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top