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Sensors 2016, 16(10), 1699; doi:10.3390/s16101699

A Graphene Oxide-Based Fluorescent Method for the Detection of Human Chorionic Gonadotropin

1
,
2
and
1,2,*
1
College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China
2
Henan Province of Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China
*
Author to whom correspondence should be addressed.
Academic Editor: Huangxian Ju
Received: 8 August 2016 / Revised: 27 September 2016 / Accepted: 29 September 2016 / Published: 13 October 2016
(This article belongs to the Special Issue Nanobiosensing for Sensors)
View Full-Text   |   Download PDF [2004 KB, uploaded 13 October 2016]   |  

Abstract

Human chorionic gonadotropin (hCG) has been regarded as a biomarker for the diagnosis of pregnancy and some cancers. Because the currently used methods (e.g., disposable Point of Care Testing (POCT) device) for hCG detection require the use of many less stable antibodies, simple and cost-effective methods for the sensitive and selective detection of hCG have always been desired. In this work, we have developed a graphene oxide (GO)-based fluorescent platform for the detection of hCG using a fluorescein isothiocyanate (FITC)-labeled hCG-specific binding peptide aptamer (denoted as FITC-PPLRINRHILTR) as the probe, which can be manufactured cheaply and consistently. Specifically, FITC-PPLRINRHILTR adsorbed onto the surface of GO via electrostatic interaction showed a poor fluorescence signal. The specific binding of hCG to FITC-PPLRINRHILTR resulted in the release of the peptide from the GO surface. As a result, an enhanced fluorescence signal was observed. The fluorescence intensity was directly proportional to the hCG concentration in the range of 0.05–20 IU/mL. The detection limit was found to be 20 mIU/mL. The amenability of the strategy to hCG analysis in biological fluids was demonstrated by assaying hCG in the urine samples. View Full-Text
Keywords: graphene oxide; fluorescent biosensors; peptide aptamer; human chorionic gonadotropin; antibody-free graphene oxide; fluorescent biosensors; peptide aptamer; human chorionic gonadotropin; antibody-free
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Xia, N.; Wang, X.; Liu, L. A Graphene Oxide-Based Fluorescent Method for the Detection of Human Chorionic Gonadotropin. Sensors 2016, 16, 1699.

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