Next Article in Journal
Monitoring Heritage Buildings with Open Source Hardware Sensors: A Case Study of the Mosque-Cathedral of Córdoba
Next Article in Special Issue
Comparison of Ultrasonic Welding and Thermal Bonding for the Integration of Thin Film Metal Electrodes in Injection Molded Polymeric Lab-on-Chip Systems for Electrochemistry
Previous Article in Journal
Ensemble of One-Class Classifiers for Personal Risk Detection Based on Wearable Sensor Data
Previous Article in Special Issue
Combined Dielectrophoresis and Impedance Systems for Bacteria Analysis in Microfluidic On-Chip Platforms
Article Menu

Export Article

Open AccessArticle
Sensors 2016, 16(10), 1616; doi:10.3390/s16101616

Arbitrarily Accessible 3D Microfluidic Device for Combinatorial High-Throughput Drug Screening

Department of Electrical Engineering, Pennsylvania State University, University Park, PA 16802, USA
Department of Entomology, Pennsylvania State University, University Park, PA 16802, USA
Department of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802, USA
Author to whom correspondence should be addressed.
Academic Editors: Amine Miled and Jesse Greener
Received: 26 August 2016 / Revised: 19 September 2016 / Accepted: 23 September 2016 / Published: 29 September 2016
(This article belongs to the Special Issue Microfluidics-Based Microsystem Integration Research)
View Full-Text   |   Download PDF [2677 KB, uploaded 29 September 2016]   |  


Microfluidics-based drug-screening systems have enabled efficient and high-throughput drug screening, but their routine uses in ordinary labs are limited due to the complexity involved in device fabrication and system setup. In this work, we report an easy-to-use and low-cost arbitrarily accessible 3D microfluidic device that can be easily adopted by various labs to perform combinatorial assays for high-throughput drug screening. The device is capable of precisely performing automatic and simultaneous reagent loading and aliquoting tasks and performing multistep assays with arbitrary sequences. The device is not intended to compete with other microfluidic technologies regarding ultra-low reaction volume. Instead, its freedom from tubing or pumping systems and easy operation makes it an ideal platform for routine high-throughput drug screening outside traditional microfluidic labs. The functionality and quantitative reliability of the 3D microfluidic device were demonstrated with a histone acetyltransferase-based drug-screening assay using the recombinant Plasmodium falciparum GCN5 enzyme, benchmarked with a traditional microtiter plate-based method. This arbitrarily accessible, multistep capable, low-cost, and easy-to-use device can be widely adopted in various combinatorial assays beyond high-throughput drug screening. View Full-Text
Keywords: microfluidic; high-throughput; combinatorial; multiplex; drug screening microfluidic; high-throughput; combinatorial; multiplex; drug screening

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Chen, Z.; Li, W.; Choi, G.; Yang, X.; Miao, J.; Cui, L.; Guan, W. Arbitrarily Accessible 3D Microfluidic Device for Combinatorial High-Throughput Drug Screening. Sensors 2016, 16, 1616.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top