Next Article in Journal
Estimation of Energy Expenditure Using a Patch-Type Sensor Module with an Incremental Radial Basis Function Neural Network
Previous Article in Journal
ReliefF-Based EEG Sensor Selection Methods for Emotion Recognition
Article Menu

Export Article

Open AccessArticle
Sensors 2016, 16(10), 1561; doi:10.3390/s16101561

A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation

1
School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China
2
School of Pharmacy, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 4 May 2016 / Revised: 24 June 2016 / Accepted: 6 July 2016 / Published: 22 September 2016
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [1720 KB, uploaded 22 September 2016]   |  

Abstract

MoS2 nanomaterial has unique properties, including innate affinity with ss-DNA and quenching ability for fluorescence dyes. Here, we present the development of a simple fluorescence biosensor based on water-soluble MoS2 nanosheets and restriction endonuclease BstUI for methylation analysis of p16 promoter. The biosensing platform exhibited excellent sensitivity in detecting DNA with a linear range of 100 pM~20 nM and a detection limit of 140 pM. More importantly, our method could distinguish as low as 1% difference in methylation level. Compared with previous methylation analysis, our design is both time saving and simple to operate, avoiding the limitations of PCR-based assays without compromising performance. View Full-Text
Keywords: MoS2; fluorescence biosensor; homogeneous analysis; DNA methylation MoS2; fluorescence biosensor; homogeneous analysis; DNA methylation
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Xiao, L.; Xu, L.; Gao, C.; Zhang, Y.; Yao, Q.; Zhang, G.-J. A MoS2 Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation. Sensors 2016, 16, 1561.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top