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Sensors 2016, 16(1), 96; doi:10.3390/s16010096

Use of Piezoelectric Immunosensors for Detection of Interferon-Gamma Interaction with Specific Antibodies in the Presence of Released-Active Forms of Antibodies to Interferon-Gamma

1
OOO “NPF “MATERIA MEDICA HOLDING”, 3rd Samotyochny per., 9, 127473 Moscow, Russia
2
Lipetsk State Technical University, Moskovskaya ul, 30, 398006 Lipetsk, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Frances S. Ligler
Received: 18 December 2015 / Accepted: 6 January 2016 / Published: 20 January 2016
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [542 KB, uploaded 21 January 2016]   |  

Abstract

In preliminary ELISA studies where released-active forms (RAF) of antibodies (Abs) to interferon-gamma (IFNg) were added to the antigen-antibody system, a statistically significant difference in absorbance signals obtained in their presence in comparison to placebo was observed. A piezoelectric immunosensor assay was developed to support these data and investigate the effects of RAF Abs to IFNg on the specific interaction between Abs to IFNg and IFNg. The experimental conditions were designed and optimal electrode coating, detection circumstances and suitable chaotropic agents for electrode regeneration were selected. The developed technique was found to provide high repeatability, intermediate precision and specificity. The difference between the analytical signals of RAF Ab samples and those of the placebo was up to 50.8%, whereas the difference between non-specific controls and the placebo was within 5%–6%. Thus, the piezoelectric immunosensor as well as ELISA has the potential to be used for detecting the effects of RAF Abs to IFNg on the antigen-antibody interaction, which might be the result of RAF’s ability to modify the affinity of IFNg to specific/related Abs. View Full-Text
Keywords: released-active forms of antibodies; piezoelectric immunosensors; interferon-gamma released-active forms of antibodies; piezoelectric immunosensors; interferon-gamma
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Don, E.; Farafonova, O.; Pokhil, S.; Barykina, D.; Nikiforova, M.; Shulga, D.; Borshcheva, A.; Tarasov, S.; Ermolaeva, T.; Epstein, O. Use of Piezoelectric Immunosensors for Detection of Interferon-Gamma Interaction with Specific Antibodies in the Presence of Released-Active Forms of Antibodies to Interferon-Gamma. Sensors 2016, 16, 96.

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