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Sensors 2015, 15(9), 22941-22955; doi:10.3390/s150922941

An Electrochemical Impedance Spectroscopy-Based Technique to Identify and Quantify Fermentable Sugars in Pineapple Waste Valorization for Bioethanol Production

1
Instituto de Ingeniería de Alimentos para el Desarrollo (IIAD), Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain
2
Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universitat Politècnica de València, Universitat de València, Camí de Vera s/n, 46022 Valencia, Spain
*
Authors to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 1 July 2015 / Revised: 31 August 2015 / Accepted: 8 September 2015 / Published: 11 September 2015
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [1571 KB, uploaded 11 September 2015]   |  

Abstract

Electrochemical Impedance Spectroscopy (EIS) has been used to develop a methodology able to identify and quantify fermentable sugars present in the enzymatic hydrolysis phase of second-generation bioethanol production from pineapple waste. Thus, a low-cost non-destructive system consisting of a stainless double needle electrode associated to an electronic equipment that allows the implementation of EIS was developed. In order to validate the system, different concentrations of glucose, fructose and sucrose were added to the pineapple waste and analyzed both individually and in combination. Next, statistical data treatment enabled the design of specific Artificial Neural Networks-based mathematical models for each one of the studied sugars and their respective combinations. The obtained prediction models are robust and reliable and they are considered statistically valid (CCR% > 93.443%). These results allow us to introduce this EIS-based technique as an easy, fast, non-destructive, and in-situ alternative to the traditional laboratory methods for enzymatic hydrolysis monitoring. View Full-Text
Keywords: bioethanol; saccharification; electrochemical impedance spectroscopy; fermentable sugars; pineapple waste bioethanol; saccharification; electrochemical impedance spectroscopy; fermentable sugars; pineapple waste
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Conesa, C.; García-Breijo, E.; Loeff, E.; Seguí, L.; Fito, P.; Laguarda-Miró, N. An Electrochemical Impedance Spectroscopy-Based Technique to Identify and Quantify Fermentable Sugars in Pineapple Waste Valorization for Bioethanol Production. Sensors 2015, 15, 22941-22955.

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