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Sensors 2015, 15(9), 22737-22749; doi:10.3390/s150922737

Development of Ionic Liquid Modified Disposable Graphite Electrodes for Label-Free Electrochemical Detection of DNA Hybridization Related to Microcystis spp.

Faculty of Pharmacy, Analytical Chemistry department, Ege University, Bornova, 35100 Izmir, Turkey
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Kagan Kerman
Received: 19 June 2015 / Revised: 20 August 2015 / Accepted: 31 August 2015 / Published: 9 September 2015
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Abstract

In this present study, ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate (IL)) modified pencil graphite electrode (IL-PGEs) was developed for electrochemical monitoring of DNA hybridization related to Microcystis spp. (MYC). The characterization of IL-PGEs was performed using microscopic and electrochemical techniques. DNA hybridization related to MYC was then explored at the surface of IL-PGEs using differential pulse voltammetry (DPV) technique. After the experimental parameters were optimized, the sequence-selective DNA hybridization related to MYC was performed in the case of hybridization between MYC probe and its complementary DNA target, noncomplementary (NC) or mismatched DNA sequence (MM), or and in the presence of mixture of DNA target: NC (1:1) and DNA target: MM (1:1). View Full-Text
Keywords: ionic liquid; nucleic acid hybridization; Microcystis spp.; electrochemical DNA biosensor; pencil graphite electrode ionic liquid; nucleic acid hybridization; Microcystis spp.; electrochemical DNA biosensor; pencil graphite electrode
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Sengiz, C.; Congur, G.; Erdem, A. Development of Ionic Liquid Modified Disposable Graphite Electrodes for Label-Free Electrochemical Detection of DNA Hybridization Related to Microcystis spp.. Sensors 2015, 15, 22737-22749.

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