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Sensors 2015, 15(9), 22291-22303; doi:10.3390/s150922291

An Apta-Biosensor for Colon Cancer Diagnostics

1
Department of Nanobiotechnology, School of New Sciences and Technologies, University of Tehran, Tehran 14395-1561, Iran
2
Department of Biotechnology and Pharmacy Engineering, Faculty of Chemical Engineering, University of Tehran, Tehran 4563-11155, Iran
3
Research Center of New Technologies in Life Science Engineering, University of Tehran, Tehran 1417963891, Iran
4
Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran 1419963111, Iran
5
Department of Electrical Engineering and Computer Science, York University, Toronto, ON M3J1P3, Canada
*
Authors to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 18 May 2015 / Revised: 19 August 2015 / Accepted: 26 August 2015 / Published: 3 September 2015
(This article belongs to the Special Issue Label-Free Sensing)
View Full-Text   |   Download PDF [2337 KB, uploaded 8 September 2015]   |  

Abstract

This paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are used in this study to demonstrate the functionality of aptasensor for colon cancer detection purposes. Flow cytometry, fluorescence microscopy and electrochemical cyclic voltammetry are used to verify the binding between the target cells and aptamer. The limit of detection (LOD) of this aptasensor is equal to seven cancer cells. Based on the experimental results, the proposed sensor can be employed for point-of-care cancer disease diagnostics. View Full-Text
Keywords: aptasensor; aptamer; cyclic voltammetry (CV); colon cancer; biosensor; fluorescence labeling aptasensor; aptamer; cyclic voltammetry (CV); colon cancer; biosensor; fluorescence labeling
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Raji, M.A.; Amoabediny, G.; Tajik, P.; Hosseini, M.; Ghafar-Zadeh, E. An Apta-Biosensor for Colon Cancer Diagnostics. Sensors 2015, 15, 22291-22303.

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