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Sensors 2015, 15(9), 21746-21759; doi:10.3390/s150921746

Time-Resolved Synchronous Fluorescence for Biomedical Diagnosis

1,2,†,* , 3
and
2,3,4,†,*
1
Department of Radiology, Duke University Medical Center, Durham, NC 27710, USA
2
Fitzpatrick Institution for Photonics, Duke University, Durham, NC 27708, USA
3
Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
4
Department of Chemistry, Duke University, Durham, NC 27708, USA
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Gonzalo Pajares Martinsanz
Received: 28 July 2015 / Revised: 24 August 2015 / Accepted: 26 August 2015 / Published: 31 August 2015
(This article belongs to the Special Issue Imaging: Sensors and Technologies)
View Full-Text   |   Download PDF [1328 KB, uploaded 3 September 2015]   |  

Abstract

This article presents our most recent advances in synchronous fluorescence (SF) methodology for biomedical diagnostics. The SF method is characterized by simultaneously scanning both the excitation and emission wavelengths while keeping a constant wavelength interval between them. Compared to conventional fluorescence spectroscopy, the SF method simplifies the emission spectrum while enabling greater selectivity, and has been successfully used to detect subtle differences in the fluorescence emission signatures of biochemical species in cells and tissues. The SF method can be used in imaging to analyze dysplastic cells in vitro and tissue in vivo. Based on the SF method, here we demonstrate the feasibility of a time-resolved synchronous fluorescence (TRSF) method, which incorporates the intrinsic fluorescent decay characteristics of the fluorophores. Our prototype TRSF system has clearly shown its advantage in spectro-temporal separation of the fluorophores that were otherwise difficult to spectrally separate in SF spectroscopy. We envision that our previously-tested SF imaging and the newly-developed TRSF methods will combine their proven diagnostic potentials in cancer diagnosis to further improve the efficacy of SF-based biomedical diagnostics. View Full-Text
Keywords: synchronous fluorescence; ultrafast; time-resolved; imaging; cancer diagnosis synchronous fluorescence; ultrafast; time-resolved; imaging; cancer diagnosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhang, X.; Fales, A.; Vo-Dinh, T. Time-Resolved Synchronous Fluorescence for Biomedical Diagnosis. Sensors 2015, 15, 21746-21759.

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