Next Article in Journal
FBG Interrogation Method with High Resolution and Response Speed Based on a Reflective-Matched FBG Scheme
Previous Article in Journal
HyperCube: A Small Lensless Position Sensing Device for the Tracking of Flickering Infrared LEDs
Article Menu

Export Article

Open AccessArticle
Sensors 2015, 15(7), 16503-16515; doi:10.3390/s150716503

Application of 3D Printing Technology in Increasing the Diagnostic Performance of Enzyme-Linked Immunosorbent Assay (ELISA) for Infectious Diseases

1
Department of Intelligent Mechanical Systems, Graduate School of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo 191-0065, Japan
2
Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan
3
Department of Microbiology, Hue University of Medicine and Pharmacy, 6 Ngo Quyen St., Hue 47000, Vietnam
4
Department of Hygiene and Public Health, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 23 May 2015 / Revised: 24 June 2015 / Accepted: 6 July 2015 / Published: 8 July 2015
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [1792 KB, uploaded 8 July 2015]   |  

Abstract

Enzyme-linked Immunosorbent Assay (ELISA)-based diagnosis is the mainstay for measuring antibody response in infectious diseases and to support pathogen identification of potential use in infectious disease outbreaks and clinical care of individual patients. The development of laboratory diagnostics using readily available 3D printing technologies provides a timely opportunity for further expansion of this technology into immunodetection systems. Utilizing available 3D printing platforms, a ‘3D well’ was designed and developed to have an increased surface area compared to those of 96-well plates. The ease and rapidity of the development of the 3D well prototype provided an opportunity for its rapid validation through the diagnostic performance of ELISA in infectious disease without modifying current laboratory practices for ELISA. The improved sensitivity of the 3D well of up to 2.25-fold higher compared to the 96-well ELISA provides a potential for the expansion of this technology towards miniaturization and Lab-On-a-Chip platforms to reduce time, volume of reagents and samples needed for such assays in the laboratory diagnosis of infectious and other diseases including applications in other disciplines. View Full-Text
Keywords: infectious diseases; 3D printing; rapid diagnostics; ELISA infectious diseases; 3D printing; rapid diagnostics; ELISA
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Singh, H.; Shimojima, M.; Shiratori, T.; Van An, L.; Sugamata, M.; Yang, M. Application of 3D Printing Technology in Increasing the Diagnostic Performance of Enzyme-Linked Immunosorbent Assay (ELISA) for Infectious Diseases. Sensors 2015, 15, 16503-16515.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top