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Sensors 2015, 15(3), 5884-5894; doi:10.3390/s150305884

Study of the Interaction of Trastuzumab and SKOV3 Epithelial Cancer Cells Using a Quartz Crystal Microbalance Sensor

1
Bioorganic & Biophysical Chemistry Laboratory, Linnæus University Centre for Biomaterials Chemistry, Department of Chemistry & Biomedical Sciences, Linnæus University, SE-39182 Kalmar, Sweden
2
Attana AB, Björnnäsvägen 21, SE-11419 Stockholm, Sweden
3
Department of Chemistry–BMC, Uppsala University, Box 576, SE-75123 Uppsala, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Peter Lieberzeit
Received: 15 December 2014 / Revised: 31 December 2014 / Accepted: 2 March 2015 / Published: 10 March 2015
(This article belongs to the Special Issue Mass-Sensitive Sensors Based on Biomimetic Recognition)
View Full-Text   |   Download PDF [1289 KB, uploaded 10 March 2015]   |  

Abstract

Analytical methods founded upon whole cell-based assays are of importance in early stage drug development and in fundamental studies of biomolecular recognition. Here we have studied the binding of the monoclonal antibody trastuzumab to human epidermal growth factor receptor 2 (HER2) on human ovary adenocarcinoma epithelial cancer cells (SKOV3) using quartz crystal microbalance (QCM) technology. An optimized procedure for immobilizing the cells on the chip surface was established with respect to fixation procedure and seeding density. Trastuzumab binding to the cell decorated sensor surface was studied, revealing a mean dissociation constant, KD, value of 7 ± 1 nM (standard error of the mean). This study provides a new perspective on the affinity of the antibody-receptor complex presented a more natural context compared to purified receptors. These results demonstrate the potential for using whole cell-based QCM assay in drug development, the screening of HER2 selective antibody-based drug candidates, and for the study of biomolecular recognition. This real time, label free approach for studying interactions with target receptors present in their natural environment afforded sensitive and detailed kinetic information about the binding of the analyte to the target. View Full-Text
Keywords: quartz crystal microbalance; breast cancer; cell-based biosensor; Herceptin; trastuzumab; HER2 quartz crystal microbalance; breast cancer; cell-based biosensor; Herceptin; trastuzumab; HER2
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Elmlund, L.; Käck, C.; Aastrup, T.; Nicholls, I.A. Study of the Interaction of Trastuzumab and SKOV3 Epithelial Cancer Cells Using a Quartz Crystal Microbalance Sensor. Sensors 2015, 15, 5884-5894.

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