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Sensors 2015, 15(2), 2763-2773; doi:10.3390/s150202763

Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System

1
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China
2
Department of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China
3
Department of Mechanical Engineering, University of Alberta, Edmonton, AB T6G 2G8, Canada
*
Authors to whom correspondence should be addressed.
Received: 6 December 2014 / Revised: 12 January 2015 / Accepted: 19 January 2015 / Published: 27 January 2015
(This article belongs to the Special Issue On-Chip Sensors)
View Full-Text   |   Download PDF [4110 KB, uploaded 27 January 2015]   |  

Abstract

This paper presents a microfluidics-based approach capable of continuously characterizing instantaneous Young’s modulus (Einstantaneous) and specific membrane capacitance (Cspecific membrane) of suspended single cells. In this method, cells were aspirated through a constriction channel while the cellular entry process into the constriction channel was recorded using a high speed camera and the impedance profiles at two frequencies (1 kHz and 100 kHz) were simultaneously measured by a lock-in amplifier. Numerical simulations were conducted to model cellular entry process into the constriction channel, focusing on two key parameters: instantaneous aspiration length (Linstantaneous) and transitional aspiration length (Ltransitional), which was further translated to Einstantaneous. An equivalent distribution circuit model for a cell travelling in the constriction channel was used to determine Cspecific membrane. A non-small-cell lung cancer cell line 95C (n = 354) was used to evaluate this technique, producing Einstantaneous of 2.96 ± 0.40 kPa and Cspecific membrane of 1.59 ± 0.28 μF/cm2. As a platform for continuous and simultaneous characterization of cellular Einstantaneous and Cspecific membrane, this approach can facilitate a more comprehensive understanding of cellular biophysical properties. View Full-Text
Keywords: microfluidics; single-cell analysis; cellular biophysics; instantaneous Young’s modulus; specific membrane capacitance microfluidics; single-cell analysis; cellular biophysics; instantaneous Young’s modulus; specific membrane capacitance
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhao, Y.; Chen, D.; Luo, Y.; Chen, F.; Zhao, X.; Jiang, M.; Yue, W.; Long, R.; Wang, J.; Chen, J. Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System. Sensors 2015, 15, 2763-2773.

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