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Sensors 2015, 15(12), 30704-30715; doi:10.3390/s151229824

Preconcentration and Separation of Mixed-Species Samples Near a Nano-Junction in a Convergent Microchannel

Department of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan
Medical Device Innovation Center, National Cheng Kung University, Tainan 70101, Taiwan
Author to whom correspondence should be addressed.
Academic Editor: Stefano Mariani
Received: 11 October 2015 / Accepted: 3 December 2015 / Published: 5 December 2015
(This article belongs to the Section Physical Sensors)
View Full-Text   |   Download PDF [1815 KB, uploaded 5 December 2015]   |  


A fluidic microchip incorporating a convergent microchannel and a Nafion-nanoporous membrane is proposed for the preconcentration and separation of multi-species samples on a single platform. In the device, sample preconcentration is achieved by means of the ion concentration polarization effect induced at the micro/nano interface under the application of an external electric field, while species separation is achieved by exploiting the different electrophoretic mobilities of the sample components. The experimental results show that the device is capable of detecting C-reactive protein (CRP) with an initial concentration as low as 9.50 × 10−6 mg/L given a sufficient preconcentration time and driving voltage. In addition, it is shown that a mixed-species sample consisting of three negatively-charged components (bovine serum albumin (BSA), tetramethylrhodamine(TAMRA) isothiocyanate-Dextran and fluorescent polymer beads) can be separated and preconcentrated within 20 min given a driving voltage of 100 V across 1 cm microchannel in length. In general, the present results confirm the feasibility of the device for the immunoassay or detection of various multi-species samples under low concentration in the biochemical and biomedical fields. The novel device can therefore improve the detection limit of traditional medical facilities. View Full-Text
Keywords: microfluidics; preconcentration; ion concentration polarization; electrophoresis microfluidics; preconcentration; ion concentration polarization; electrophoresis

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Chiu, P.-H.; Weng, C.-H.; Yang, R.-J. Preconcentration and Separation of Mixed-Species Samples Near a Nano-Junction in a Convergent Microchannel. Sensors 2015, 15, 30704-30715.

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