Integration of a Miniature Quartz Crystal Microbalance with a Microfluidic Chip for Amyloid Beta-Aβ42 Quantitation
AbstractA miniature quartz crystal microbalance (mQCM) was integrated with a polydimethylsiloxane (PDMS) microfluidic device for on-chip determination of amyloid polypeptide–Aβ42. The integration techniques included photolithography and plasma coupling. Aβ42 antibody was immobilized on the mQCM surface using a cross-linker method, and the resonance frequency of mQCM shifted negatively due to antibody-antigen binding. A linear range from 0.1 µM to 3.2 µM was achieved. By using matrix elimination buffer, i.e., matrix phosphate buffer containing 500 µg/mL dextran and 0.5% Tween 20, Aβ42 could be successfully detected in the presence of 75% human serum. Additionally, high temperature treatments at 150 °C provided a valid method to recover mQCM, and PDMS-mQCM microfluidic device could be reused to some extent. Since the detectable Aβ42 concentration could be as low as 0.1 µM, which is close to cut-off value for Alzheimer patients, the PDMS-mQCM device could be applied in early Alzheimer’s disease diagnosis. View Full-Text
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Tao, W.; Xie, Q.; Wang, H.; Ke, S.; Lin, P.; Zeng, X. Integration of a Miniature Quartz Crystal Microbalance with a Microfluidic Chip for Amyloid Beta-Aβ42 Quantitation. Sensors 2015, 15, 25746-25760.
Tao W, Xie Q, Wang H, Ke S, Lin P, Zeng X. Integration of a Miniature Quartz Crystal Microbalance with a Microfluidic Chip for Amyloid Beta-Aβ42 Quantitation. Sensors. 2015; 15(10):25746-25760.Chicago/Turabian Style
Tao, Wenyan; Xie, Qingji; Wang, Hairui; Ke, Shanming; Lin, Peng; Zeng, Xierong. 2015. "Integration of a Miniature Quartz Crystal Microbalance with a Microfluidic Chip for Amyloid Beta-Aβ42 Quantitation." Sensors 15, no. 10: 25746-25760.