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Sensors 2015, 15(10), 25746-25760; doi:10.3390/s151025746

Integration of a Miniature Quartz Crystal Microbalance with a Microfluidic Chip for Amyloid Beta-Aβ42 Quantitation

1
Shenzhen Key Laboratory of Special Functional Materials & Shenzhen Engineering Laboratory for Advance Technology of Ceramics, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, China
2
College of Electronic Science and Technology, Shenzhen University, Shenzhen 518060, China
3
College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Kagan Kerman
Received: 24 June 2015 / Revised: 18 September 2015 / Accepted: 22 September 2015 / Published: 12 October 2015
View Full-Text   |   Download PDF [1291 KB, uploaded 12 October 2015]   |  

Abstract

A miniature quartz crystal microbalance (mQCM) was integrated with a polydimethylsiloxane (PDMS) microfluidic device for on-chip determination of amyloid polypeptide–Aβ42. The integration techniques included photolithography and plasma coupling. Aβ42 antibody was immobilized on the mQCM surface using a cross-linker method, and the resonance frequency of mQCM shifted negatively due to antibody-antigen binding. A linear range from 0.1 µM to 3.2 µM was achieved. By using matrix elimination buffer, i.e., matrix phosphate buffer containing 500 µg/mL dextran and 0.5% Tween 20, Aβ42 could be successfully detected in the presence of 75% human serum. Additionally, high temperature treatments at 150 °C provided a valid method to recover mQCM, and PDMS-mQCM microfluidic device could be reused to some extent. Since the detectable Aβ42 concentration could be as low as 0.1 µM, which is close to cut-off value for Alzheimer patients, the PDMS-mQCM device could be applied in early Alzheimer’s disease diagnosis. View Full-Text
Keywords: integration; miniature quartz crystal microbalance; polydimethylsiloxane; amyloid polypeptide–Aβ42 integration; miniature quartz crystal microbalance; polydimethylsiloxane; amyloid polypeptide–Aβ42
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tao, W.; Xie, Q.; Wang, H.; Ke, S.; Lin, P.; Zeng, X. Integration of a Miniature Quartz Crystal Microbalance with a Microfluidic Chip for Amyloid Beta-Aβ42 Quantitation. Sensors 2015, 15, 25746-25760.

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