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Sensors 2014, 14(9), 16785-16798; doi:10.3390/s140916785

Development of an Immunochromatographic Strip Test for Rapid Detection of Ciprofloxacin in Milk Samples

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
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Author to whom correspondence should be addressed.
Received: 30 June 2014 / Revised: 20 August 2014 / Accepted: 5 September 2014 / Published: 10 September 2014
(This article belongs to the Special Issue Nanoparticle-Based Biosensors)
View Full-Text   |   Download PDF [475 KB, uploaded 11 September 2014]   |  

Abstract

A rapid, simple, and sensitive immunochromatographic test strip has been developed for testing residues of ciprofloxacin (CIP). A specific and sensitive monoclonal antibody (mAb) for CIP was generated by immunizing BALB/c mice with well-characterized CIP-Keyhole limpet haemocyanin. Under the optimized conditions, the cut-off limits of test strips for CIP were found to be 5 ng/mL in phosphate-buffered saline and 2.5 ng/mL in milk samples. Each test can be evaluated within 3 min. The cross-reactivities of the CIP test strip to enrofloxacin (ENR), norfloxacin (NOR), nadifloxacin (NDF), danofloxacin (DANO), pefloxacin (PEX), lomefloxacin (LOME), enoxacin (ENO), and sarafloxacin (SAR) were 71.4%, 71.4%, 66%, 50%, 33%, 20%, 12.5%, and 6.25%, respectively. The data indicate that the method is sensitive, specific, and has the advantages of simplicity and speed, therefore, this test strip is a useful screening method for the detection of CIP residues in milk samples. View Full-Text
Keywords: ciprofloxacin; immunochromatographic assay; test strip; monoclonal antibody ciprofloxacin; immunochromatographic assay; test strip; monoclonal antibody
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Liu, L.; Luo, L.; Suryoprabowo, S.; Peng, J.; Kuang, H.; Xu, C. Development of an Immunochromatographic Strip Test for Rapid Detection of Ciprofloxacin in Milk Samples. Sensors 2014, 14, 16785-16798.

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