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Sensors 2014, 14(7), 12059-12069; doi:10.3390/s140712059
Article

An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol

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Received: 26 May 2014; in revised form: 20 June 2014 / Accepted: 26 June 2014 / Published: 7 July 2014
(This article belongs to the Section Biosensors)
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Abstract: We report on the aptadetection of chloramphenicol (CAP) using electrochemical impedance spectroscopy. The detection principle is based on the changes of the interfacial properties of the electrode after the interaction of the ssDNA aptamers with the target molecules. The electrode surface is partially blocked due to the formation of the aptamer-CAP complex, resulting in an increase of the interfacial electron-transfer resistance of the redox probe detected by electrochemical impedance spectroscopy or cyclic voltammetry. We observed that the ratio of polarization resistance had a linear relationship with the concentrations of CAP in the range of 1.76–127 nM, and a detection limit of 1.76 nM was obtained. The covalent binding of CAP-aptamer on the electrode surface combined with the unique properties of aptamers and impedimetric transduction leads to the development of a stable and sensitive electrochemical aptasensor for CAP.
Keywords: aptasensor; electrochemical impedance spectroscopy; chloramphenicol; specific recognition aptasensor; electrochemical impedance spectroscopy; chloramphenicol; specific recognition
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Pilehvar, S.; Dierckx, T.; Blust, R.; Breugelmans, T.; De Wael, K. An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol. Sensors 2014, 14, 12059-12069.

AMA Style

Pilehvar S, Dierckx T, Blust R, Breugelmans T, De Wael K. An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol. Sensors. 2014; 14(7):12059-12069.

Chicago/Turabian Style

Pilehvar, Sanaz; Dierckx, Tarryn; Blust, Ronny; Breugelmans, Tom; De Wael, Karolien. 2014. "An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol." Sensors 14, no. 7: 12059-12069.



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