Quorum Sensing Activity of Aeromonas Caviae Strain YL12, A Bacterium Isolated from Compost
AbstractQuorum sensing is a well-studied cell-to-cell communication method that involves a cell-density dependent regulation of genes expression mediated by signalling molecules. In this study, a bacterium isolated from a plant material compost pile was found to possess quorum sensing activity based on bioassay screening. Isolate YL12 was identified using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and molecular typing using rpoD gene which identified the isolate as Aeromonas caviae. High resolution tandem mass spectrometry was subsequently employed to identify the N-acyl homoserine lactone profile of Aeromonas caviae YL12 and confirmed that this isolate produced two short chain N-acyl homoserine lactones, namely C4-HSL and C6, and the production was observed to be cell density-dependent. Using the thin layer chromatography (TLC) bioassay, both AHLs were found to activate C. violaceum CV026, whereas only C6-HSL was revealed to induce bioluminescence expression of E. coli [pSB401]. The data presented in this study will be the leading steps in understanding the role of quorum sensing in Aeromonas caviae strain YL12. View Full-Text
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Lim, Y.-L.; Ee, R.; Yin, W.-F.; Chan, K.-G. Quorum Sensing Activity of Aeromonas Caviae Strain YL12, A Bacterium Isolated from Compost. Sensors 2014, 14, 7026-7040.
Lim Y-L, Ee R, Yin W-F, Chan K-G. Quorum Sensing Activity of Aeromonas Caviae Strain YL12, A Bacterium Isolated from Compost. Sensors. 2014; 14(4):7026-7040.Chicago/Turabian Style
Lim, Yan-Lue; Ee, Robson; Yin, Wai-Fong; Chan, Kok-Gan. 2014. "Quorum Sensing Activity of Aeromonas Caviae Strain YL12, A Bacterium Isolated from Compost." Sensors 14, no. 4: 7026-7040.