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Sensors 2014, 14(4), 6695-6700; doi:10.3390/s140406695
Communication

Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method

1,2,* , 1,2
,
1
,
1,2
,
1
 and
1,2
1 Group of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, Spain 2 Applied Physics Department, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, José Gutiérrez Abascal, 2, 28006 Madrid, Spain
* Author to whom correspondence should be addressed.
Received: 29 December 2013 / Revised: 10 March 2014 / Accepted: 3 April 2014 / Published: 10 April 2014
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Abstract

In this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The blocking reagent used for this optimization is a dry milk diluted in phosphate buffered saline. The recognition curve of dengue virus over the proposed surface sensor demonstrates the capacity of this method to be applied in Point of Care technology.
Keywords: photonic sensing cells; biosensors; label-free immunoassay; dengue virus photonic sensing cells; biosensors; label-free immunoassay; dengue virus
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Laguna, M.F.; Holgado, M.; Sanza, F.J.; Lavín, A.; López, A.; Casquel, R. Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method. Sensors 2014, 14, 6695-6700.

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