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Open AccessCommunication
Sensors 2014, 14(4), 6695-6700; doi:10.3390/s140406695

Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method

Group of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, Spain
Applied Physics Department, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, José Gutiérrez Abascal, 2, 28006 Madrid, Spain
Author to whom correspondence should be addressed.
Received: 29 December 2013 / Revised: 10 March 2014 / Accepted: 3 April 2014 / Published: 10 April 2014
(This article belongs to the Special Issue Photonic Sensors for Industrial, Environmental and Health Monitoring)
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In this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The blocking reagent used for this optimization is a dry milk diluted in phosphate buffered saline. The recognition curve of dengue virus over the proposed surface sensor demonstrates the capacity of this method to be applied in Point of Care technology.
Keywords: photonic sensing cells; biosensors; label-free immunoassay; dengue virus photonic sensing cells; biosensors; label-free immunoassay; dengue virus

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Laguna, M.F.; Holgado, M.; Sanza, F.J.; Lavín, A.; López, A.; Casquel, R. Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method. Sensors 2014, 14, 6695-6700.

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