Sensors 2014, 14(4), 6695-6700; doi:10.3390/s140406695
Communication

Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method

1,2,* email, 1,2email, 1email, 1,2email, 1email and 1,2email
Received: 29 December 2013; in revised form: 10 March 2014 / Accepted: 3 April 2014 / Published: 10 April 2014
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The blocking reagent used for this optimization is a dry milk diluted in phosphate buffered saline. The recognition curve of dengue virus over the proposed surface sensor demonstrates the capacity of this method to be applied in Point of Care technology.
Keywords: photonic sensing cells; biosensors; label-free immunoassay; dengue virus
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MDPI and ACS Style

Laguna, M.F.; Holgado, M.; Sanza, F.J.; Lavín, A.; López, A.; Casquel, R. Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method. Sensors 2014, 14, 6695-6700.

AMA Style

Laguna MF, Holgado M, Sanza FJ, Lavín A, López A, Casquel R. Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method. Sensors. 2014; 14(4):6695-6700.

Chicago/Turabian Style

Laguna, María F.; Holgado, Miguel; Sanza, Francisco J.; Lavín, Alvaro; López, Ana; Casquel, Rafael. 2014. "Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method." Sensors 14, no. 4: 6695-6700.

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