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Sensors 2014, 14(3), 5611-5621; doi:10.3390/s140305611

Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus

3,4,*  and 1,*
1 Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350005, China 2 Department of Laboratory Medicines, Fujian Medical University, Fuzhou 350004, China 3 Department of Laboratory Medicine, the First Affiliated Hospital, Fujian Medical University, Fuzhou 35000, China 4 Department of Laboratory Medicine, Medical Technology and Engineering College, Fujian Medical University, Fuzhou 35000, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 8 July 2013 / Revised: 22 July 2013 / Accepted: 29 August 2013 / Published: 20 March 2014
(This article belongs to the Section Biosensors)
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Objective: To develop a convenient; sensitive; accurate; and economical technique for genotyping of hepatitis B viruses (HBVs). Methods: The mercapto-modified B1; B2; C1; and C2-specific genotyping probes consisted of two probes for each HBV genotype that served as a double verification system. These probes were fixed on the surface of No. 1; 2; 3; and 4 gold electrodes; respectively; via Au-S bonds. Different charge generated by the binding of RuHex to phosphate groups of the DNA backbone before and after hybridization was used for distinguishing the different genotypes. Results: During hybridization with genotype B; the charges detected at the No. 1 and 2 electrodes were significantly increased; while the charge at the No. 3 and 4 electrodes did not change significantly. During hybridization with genotype C; the charges detected at No. 3 and 4 electrodes were significantly increased; while the signals remained unchanged at the No. 1 and 2 electrodes. During hybridization with mixed genotypes (B and C); the charges detected at all four electrodes were significantly increased. The linear range of detection was 10–7 to 10–10 mol/L and the sensitivity for detecting mixed B (10%) or C (10%). Conclusions: Rapid genotyping of HBVs based on electrochemical sensing is simple, has good specificity; and can greatly reduce the cost. This method can be used for sensitive detection of mixed B and C HBV genotypes.
Keywords: HBV genotypes; biosensors; RuHex HBV genotypes; biosensors; RuHex
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chen, J.; Weng, S.; Chen, Q.; Liu, A.; Wang, F.; Chen, J.; Yi, Q.; Liu, Q.; Lin, X. Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus. Sensors 2014, 14, 5611-5621.

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