Abstract: Objective: To develop a convenient; sensitive; accurate; and economical technique for genotyping of hepatitis B viruses (HBVs). Methods: The mercapto-modified B1; B2; C1; and C2-specific genotyping probes consisted of two probes for each HBV genotype that served as a double verification system. These probes were fixed on the surface of No. 1; 2; 3; and 4 gold electrodes; respectively; via Au-S bonds. Different charge generated by the binding of RuHex to phosphate groups of the DNA backbone before and after hybridization was used for distinguishing the different genotypes. Results: During hybridization with genotype B; the charges detected at the No. 1 and 2 electrodes were significantly increased; while the charge at the No. 3 and 4 electrodes did not change significantly. During hybridization with genotype C; the charges detected at No. 3 and 4 electrodes were significantly increased; while the signals remained unchanged at the No. 1 and 2 electrodes. During hybridization with mixed genotypes (B and C); the charges detected at all four electrodes were significantly increased. The linear range of detection was 10–7 to 10–10 mol/L and the sensitivity for detecting mixed B (10%) or C (10%). Conclusions: Rapid genotyping of HBVs based on electrochemical sensing is simple, has good specificity; and can greatly reduce the cost. This method can be used for sensitive detection of mixed B and C HBV genotypes.
Keywords: HBV genotypes; biosensors; RuHex
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Chen, J.; Weng, S.; Chen, Q.; Liu, A.; Wang, F.; Chen, J.; Yi, Q.; Liu, Q.; Lin, X. Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus. Sensors 2014, 14, 5611-5621.
Chen J, Weng S, Chen Q, Liu A, Wang F, Chen J, Yi Q, Liu Q, Lin X. Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus. Sensors. 2014; 14(3):5611-5621.
Chen, Jinyuan; Weng, Shaohuang; Chen, Qingqiong; Liu, Ailin; Wang, Fengqing; Chen, Jing; Yi, Qiang; Liu, Qicai; Lin, Xinhua. 2014. "Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus." Sensors 14, no. 3: 5611-5621.