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Sensors 2014, 14(3), 4585-4598; doi:10.3390/s140304585
Article

Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications

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1 Institute of Microelectronics, Agency for Science, Technology and Research (A*STAR), Singapore 117685, Singapore 2 Department of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore 3 Department of Mechanical and Automotive Engineering, Seoul National University of Science and Technology, Seoul 139743, Korea 4 InvenSense Inc., San Jose, CA 95110, USA
* Author to whom correspondence should be addressed.
Received: 27 December 2013 / Revised: 22 February 2014 / Accepted: 26 February 2014 / Published: 7 March 2014
(This article belongs to the Section Biosensors)
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Abstract

Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The resonance frequency of the biotin modified resonator decreased 315 ppm when exposed to streptavidin solution for 15 min with a concentration of 10−7 M, corresponding to an added mass of 3.43 ng on the resonator surface. An additional control is added by exposing a bovine serum albumin (BSA)-covered device to streptavidin in the absence of the attached biotin. No resonance frequency shift was observed in the control experiment, which confirms the specificity of the detection. The sensor-to-sensor variability is also measured to be 4.3%. Consequently, the developed sensor can be used to observe in biotin-streptavidin interaction without the use of labelling or molecular tags. In addition, biosensor can be used in a variety of different immunoassay tests.
Keywords: biosensor; capacitive filled gap transducer; biotin; streptavidin biosensor; capacitive filled gap transducer; biotin; streptavidin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Heidari, A.; Yoon, Y.-J.; Park, W.-T.; Su, P.-C.; Miao, J.; Lin, J.T.M.; Park, M.K. Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications. Sensors 2014, 14, 4585-4598.

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