Sensors 2014, 14(3), 4585-4598; doi:10.3390/s140304585
Article

Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications

1,2email, 2,* email, 3email, 2email, 2email, 1,4email and 1email
Received: 27 December 2013; in revised form: 22 February 2014 / Accepted: 26 February 2014 / Published: 7 March 2014
(This article belongs to the Section Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The resonance frequency of the biotin modified resonator decreased 315 ppm when exposed to streptavidin solution for 15 min with a concentration of 10−7 M, corresponding to an added mass of 3.43 ng on the resonator surface. An additional control is added by exposing a bovine serum albumin (BSA)-covered device to streptavidin in the absence of the attached biotin. No resonance frequency shift was observed in the control experiment, which confirms the specificity of the detection. The sensor-to-sensor variability is also measured to be 4.3%. Consequently, the developed sensor can be used to observe in biotin-streptavidin interaction without the use of labelling or molecular tags. In addition, biosensor can be used in a variety of different immunoassay tests.
Keywords: biosensor; capacitive filled gap transducer; biotin; streptavidin
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MDPI and ACS Style

Heidari, A.; Yoon, Y.-J.; Park, W.-T.; Su, P.-C.; Miao, J.; Lin, J.T.M.; Park, M.K. Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications. Sensors 2014, 14, 4585-4598.

AMA Style

Heidari A, Yoon Y-J, Park W-T, Su P-C, Miao J, Lin JTM, Park MK. Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications. Sensors. 2014; 14(3):4585-4598.

Chicago/Turabian Style

Heidari, Amir; Yoon, Yong-Jin; Park, Woo-Tae; Su, Pei-Chen; Miao, Jianmin; Lin, Julius T.M.; Park, Mi K. 2014. "Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications." Sensors 14, no. 3: 4585-4598.

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