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Sensors 2014, 14(3), 4585-4598; doi:10.3390/s140304585

Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications

Institute of Microelectronics, Agency for Science, Technology and Research (A*STAR), Singapore 117685, Singapore
Department of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore
Department of Mechanical and Automotive Engineering, Seoul National University of Science and Technology, Seoul 139743, Korea
InvenSense Inc., San Jose, CA 95110, USA
Author to whom correspondence should be addressed.
Received: 27 December 2013 / Revised: 22 February 2014 / Accepted: 26 February 2014 / Published: 7 March 2014
(This article belongs to the Section Biosensors)
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Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The resonance frequency of the biotin modified resonator decreased 315 ppm when exposed to streptavidin solution for 15 min with a concentration of 10−7 M, corresponding to an added mass of 3.43 ng on the resonator surface. An additional control is added by exposing a bovine serum albumin (BSA)-covered device to streptavidin in the absence of the attached biotin. No resonance frequency shift was observed in the control experiment, which confirms the specificity of the detection. The sensor-to-sensor variability is also measured to be 4.3%. Consequently, the developed sensor can be used to observe in biotin-streptavidin interaction without the use of labelling or molecular tags. In addition, biosensor can be used in a variety of different immunoassay tests. View Full-Text
Keywords: biosensor; capacitive filled gap transducer; biotin; streptavidin biosensor; capacitive filled gap transducer; biotin; streptavidin

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Heidari, A.; Yoon, Y.-J.; Park, W.-T.; Su, P.-C.; Miao, J.; Lin, J.T.M.; Park, M.K. Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications. Sensors 2014, 14, 4585-4598.

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