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Sensors 2014, 14(10), 19128-19137; doi:10.3390/s141019128

Comparison between a Conductometric Biosensor and ELISA in the Evaluation of Johne’s Disease

1
Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824, USA
2
Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA
3
Diagnostic Center for Population and Animal Health, Michigan State University, East Lansing, MI 48824, USA
*
Author to whom correspondence should be addressed.
Received: 11 August 2014 / Revised: 8 October 2014 / Accepted: 9 October 2014 / Published: 15 October 2014
(This article belongs to the Special Issue Biosensors for Pathogen Detection)
View Full-Text   |   Download PDF [842 KB, uploaded 15 October 2014]   |  

Abstract

Johne’s disease (JD), caused by Mycobacterium avium subspecies paratuberculosis (MAP), is an important gastrointestinal disease of cattle worldwide because of the economic losses encountered in JD-affected herds. These losses include reduction in milk yield in cows, premature culling and reduced carcass weight of culled diseased animals. In the U.S. dairy industry, economic losses from reduced productivity associated with JD are estimated to cost between $200 and $250 million annually. The development of non-laboratory-based assays would support more frequent testing of animals for JD and could improve its control. Conductometric biosensors combine immunomigration technology with electronic signal detection and have been adapted for the detection of IgG antibody against MAP. In the present study, a capture membrane with limited variability in the immunomigration channel and an optimal concentration of the secondary anti-bovine antibody used in a previously developed conductometric biosensor were compared with a commercially available antibody detection ELISA in their evaluation of JD, using samples of serum from cattle whose JD status where unknown. There was a moderate strength of agreement (kappa = 0.41) between the two assays. Findings from this preliminary study support the continued development of conductometric biosensors for use in the diagnosis of JD. View Full-Text
Keywords: biosensor; conductometric; diagnosis; immunosensor; Johne’s disease; paratuberculosis biosensor; conductometric; diagnosis; immunosensor; Johne’s disease; paratuberculosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Okafor, C.; Grooms, D.; Alocilja, E.; Bolin, S. Comparison between a Conductometric Biosensor and ELISA in the Evaluation of Johne’s Disease. Sensors 2014, 14, 19128-19137.

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