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Sensors 2014, 14(1), 1028-1038; doi:10.3390/s140101028

Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis

Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, Bucharest 060031, Romania
Author to whom correspondence should be addressed.
Received: 6 November 2013 / Revised: 16 December 2013 / Accepted: 24 December 2013 / Published: 9 January 2014
(This article belongs to the Section Biosensors)
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A rapid, simple and stable biosensor for aspartame detection was developed. Alcohol oxidase (AOX), carboxyl esterase (CaE) and bovine serum albumin (BSA) were immobilised with glutaraldehyde (GA) onto screen-printed electrodes modified with cobalt-phthalocyanine (CoPC). The biosensor response was fast. The sample throughput using a flow injection analysis (FIA) system was 40 h−1 with an RSD of 2.7%. The detection limits for both batch and FIA measurements were 0.1 µM for methanol and 0.2 µM for aspartame, respectively. The enzymatic biosensor was successfully applied for aspartame determination in different sample matrices/commercial products (liquid and solid samples) without any pre-treatment step prior to measurement. View Full-Text
Keywords: alcohol oxidase; carboxyl esterase; flow injection analysis; aspartame alcohol oxidase; carboxyl esterase; flow injection analysis; aspartame

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Radulescu, M.-C.; Bucur, B.; Bucur, M.-P.; Radu, G.L. Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis. Sensors 2014, 14, 1028-1038.

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