Sensors 2013, 13(12), 15898-15911; doi:10.3390/s131215898
Article

Microfluidic Biosensor Array with Integrated Poly(2,7-Carbazole)/Fullerene-Based Photodiodes for Rapid Multiplexed Detection of Pathogens

Department of Micro and Nano Systems Technology, Faculty of Technology and Maritime Sciences, Vestfold University College, Box 2243, N-3103 Tonsberg, Norway
* Author to whom correspondence should be addressed.
Received: 20 October 2013; in revised form: 10 November 2013 / Accepted: 13 November 2013 / Published: 25 November 2013
(This article belongs to the Section Biosensors)
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Abstract: A multiplexed microfluidic biosensor made of poly(methylmethacrylate) (PMMA) was integrated into an array of organic blend heterojunction photodiodes (OPDs) for chemiluminescent detection of pathogens. Waterborne Escherichia coli O157:H7, Campylobacter jejuni and adenovirus were targeted in the PMMA chip, and detection of captured pathogens was conducted by poly(2,7-carbazole)/fullerene OPDs which showed a responsivity over 0.20 A/W at 425 nm. The limits of chemiluminescent detection were 5 × 105 cells/mL for E. coli, 1 × 105 cells/mL for C. jejuni, and 1 × 10−8 mg/mL for adenovirus. Parallel analysis for all three analytes in less than 35 min was demonstrated. Further recovery tests illustrated the potential of the integrated biosensor for detecting bacteria in real water samples.
Keywords: organic photodetector; microfluidic integration; multiplexed detection; environmental monitoring; point-of-care

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MDPI and ACS Style

Matos Pires, N.M.; Dong, T. Microfluidic Biosensor Array with Integrated Poly(2,7-Carbazole)/Fullerene-Based Photodiodes for Rapid Multiplexed Detection of Pathogens. Sensors 2013, 13, 15898-15911.

AMA Style

Matos Pires NM, Dong T. Microfluidic Biosensor Array with Integrated Poly(2,7-Carbazole)/Fullerene-Based Photodiodes for Rapid Multiplexed Detection of Pathogens. Sensors. 2013; 13(12):15898-15911.

Chicago/Turabian Style

Matos Pires, Nuno M.; Dong, Tao. 2013. "Microfluidic Biosensor Array with Integrated Poly(2,7-Carbazole)/Fullerene-Based Photodiodes for Rapid Multiplexed Detection of Pathogens." Sensors 13, no. 12: 15898-15911.

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