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Sensors 2013, 13(12), 15898-15911; doi:10.3390/s131215898
Article

Microfluidic Biosensor Array with Integrated Poly(2,7-Carbazole)/Fullerene-Based Photodiodes for Rapid Multiplexed Detection of Pathogens

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Received: 20 October 2013 / Revised: 10 November 2013 / Accepted: 13 November 2013 / Published: 25 November 2013
(This article belongs to the Section Biosensors)
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Abstract

A multiplexed microfluidic biosensor made of poly(methylmethacrylate) (PMMA) was integrated into an array of organic blend heterojunction photodiodes (OPDs) for chemiluminescent detection of pathogens. Waterborne Escherichia coli O157:H7, Campylobacter jejuni and adenovirus were targeted in the PMMA chip, and detection of captured pathogens was conducted by poly(2,7-carbazole)/fullerene OPDs which showed a responsivity over 0.20 A/W at 425 nm. The limits of chemiluminescent detection were 5 × 105 cells/mL for E. coli, 1 × 105 cells/mL for C. jejuni, and 1 × 10−8 mg/mL for adenovirus. Parallel analysis for all three analytes in less than 35 min was demonstrated. Further recovery tests illustrated the potential of the integrated biosensor for detecting bacteria in real water samples.
Keywords: organic photodetector; microfluidic integration; multiplexed detection; environmental monitoring; point-of-care organic photodetector; microfluidic integration; multiplexed detection; environmental monitoring; point-of-care
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Matos Pires, N.M.; Dong, T. Microfluidic Biosensor Array with Integrated Poly(2,7-Carbazole)/Fullerene-Based Photodiodes for Rapid Multiplexed Detection of Pathogens. Sensors 2013, 13, 15898-15911.

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