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Sensors 2013, 13(11), 15242-15251; doi:10.3390/s131115242
Article

Short Chain N-Acylhomoserine Lactone Production by Clinical Multidrug Resistant Klebsiella pneumoniae Strain CSG20

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Received: 20 September 2013; in revised form: 23 October 2013 / Accepted: 31 October 2013 / Published: 7 November 2013
(This article belongs to the Section Biosensors)
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Abstract: Klebsiella pneumoniae is one of the most common Gram-negative bacterial pathogens in clinical practice. It is associated with a wide range of disorders, ranging from superficial skin and soft tissue infections to potentially fatal sepsis in the lungs and blood stream. Quorum sensing, or bacterial cell-cell communication, refers to population density-dependent gene expression modulation. Quorum sensing in Proteobacteria relies on the production and sensing of signaling molecules which are mostly N-acylhomoserine lactones. Here, we report the identification of a multidrug resistant clinical isolate, K. pneumoniae strain CSG20, using matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry. We further confirmed quorum sensing activity in this strain with the use of high resolution tandem liquid chromatography quadrupole mass spectrometry and provided evidence K. pneumoniae strain CSG20 produced N-hexanoyl-homoserine lactone (C6-HSL). To the best of our knowledge, this is the first report on the production of N-hexanoylhomoserine lactone (C6-HSL) in clinical isolate K. pneumoniae.
Keywords: Klebsiella pneumonia; multidrug resistant; mass spectrometry; MALDI-TOF; N-hexanoylhomoserine lactone (C6-HSL); clinical pathogen; quorum sensing Klebsiella pneumonia; multidrug resistant; mass spectrometry; MALDI-TOF; N-hexanoylhomoserine lactone (C6-HSL); clinical pathogen; quorum sensing
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ngeow, Y.F.; Cheng, H.J.; Chen, J.W.; Yin, W.-F.; Chan, K.-G. Short Chain N-Acylhomoserine Lactone Production by Clinical Multidrug Resistant Klebsiella pneumoniae Strain CSG20. Sensors 2013, 13, 15242-15251.

AMA Style

Ngeow YF, Cheng HJ, Chen JW, Yin W-F, Chan K-G. Short Chain N-Acylhomoserine Lactone Production by Clinical Multidrug Resistant Klebsiella pneumoniae Strain CSG20. Sensors. 2013; 13(11):15242-15251.

Chicago/Turabian Style

Ngeow, Yun F.; Cheng, Huey J.; Chen, Jian W.; Yin, Wai-Fong; Chan, Kok-Gan. 2013. "Short Chain N-Acylhomoserine Lactone Production by Clinical Multidrug Resistant Klebsiella pneumoniae Strain CSG20." Sensors 13, no. 11: 15242-15251.


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