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Sensors 2013, 13(11), 14650-14661; doi:10.3390/s131114650
Article

Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions

1,2,†,* , 3,†
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Received: 28 August 2013; in revised form: 24 September 2013 / Accepted: 3 October 2013 / Published: 29 October 2013
(This article belongs to the Section Physical Sensors)
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Abstract: In this article we describe the integration of impedance spectroscopy (EIS) and surface plasmon resonance (SPR) into one surface analytic device. A polydimethylsiloxane (PDMS) flow cell is created, matching the dimensions of a commercially available sensor chip used for SPR measurements. This flow cell allowed simultaneous measurements between an EIS and a SPR setup. After a successful integration, a proof of principle study was conducted to investigate any signs of interference between the two systems during a measurement. The flow cell was rinsed with 10 mM Tris-HCl and 1× PBS buffer in an alternating manner, while impedance and shifts of the resonance angle were monitored. After achieving a successful proof of principle, a usability test was conducted. It was assessed whether simultaneous detection occurred when: (i) Protein A is adsorbed to the gold surface of the chip; (ii) The non-occupied zone is blocked with BSA molecules and (iii) IgG1 is bound to the Protein A. The results indicate a successful merge between SPR and EIS.
Keywords: SPR; EIS; flow-cell; lab on chip; Protein A; immunoglobulin SPR; EIS; flow-cell; lab on chip; Protein A; immunoglobulin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Vandenryt, T.; Pohl, A.; van Grinsven, B.; Thoelen, R.; De Ceuninck, W.; Wagner, P.; Opitz, J. Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions. Sensors 2013, 13, 14650-14661.

AMA Style

Vandenryt T, Pohl A, van Grinsven B, Thoelen R, De Ceuninck W, Wagner P, Opitz J. Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions. Sensors. 2013; 13(11):14650-14661.

Chicago/Turabian Style

Vandenryt, Thijs; Pohl, Andrea; van Grinsven, Bart; Thoelen, Ronald; De Ceuninck, Ward; Wagner, Patrick; Opitz, Jörg. 2013. "Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions." Sensors 13, no. 11: 14650-14661.


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