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Sensors 2013, 13(11), 14650-14661; doi:10.3390/s131114650
Article

Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions

1,2,†,* , 3,†
,
1
,
1,2
,
1,4
,
1,4
 and
3
1 Institute for Materials Research IMO, Hasselt University, Wetenschapspark 1, 3590 Diepenbeek, Belgium 2 XIOS University College, Agoralaan building H, 3590 Diepenbeek, Belgium 3 Fraunhofer Institute for Non Destructive Testing (IZFP), Maria-Reiche-Strasse 2, 01109 Dresden, Germany 4 IMEC vzw, IMOMEC, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 28 August 2013 / Revised: 24 September 2013 / Accepted: 3 October 2013 / Published: 29 October 2013
(This article belongs to the Section Chemical Sensors)
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Abstract

In this article we describe the integration of impedance spectroscopy (EIS) and surface plasmon resonance (SPR) into one surface analytic device. A polydimethylsiloxane (PDMS) flow cell is created, matching the dimensions of a commercially available sensor chip used for SPR measurements. This flow cell allowed simultaneous measurements between an EIS and a SPR setup. After a successful integration, a proof of principle study was conducted to investigate any signs of interference between the two systems during a measurement. The flow cell was rinsed with 10 mM Tris-HCl and 1× PBS buffer in an alternating manner, while impedance and shifts of the resonance angle were monitored. After achieving a successful proof of principle, a usability test was conducted. It was assessed whether simultaneous detection occurred when: (i) Protein A is adsorbed to the gold surface of the chip; (ii) The non-occupied zone is blocked with BSA molecules and (iii) IgG1 is bound to the Protein A. The results indicate a successful merge between SPR and EIS.
Keywords: SPR; EIS; flow-cell; lab on chip; Protein A; immunoglobulin SPR; EIS; flow-cell; lab on chip; Protein A; immunoglobulin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Vandenryt, T.; Pohl, A.; van Grinsven, B.; Thoelen, R.; De Ceuninck, W.; Wagner, P.; Opitz, J. Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions. Sensors 2013, 13, 14650-14661.

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