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Design and Fabrication of Low-Cost 1536-Chamber Microfluidic Microarrays for Mood-Disorders-Related Serological Studies
Department of Micro and Nano Systems Technology (IMST), Faculty of Technology and Maritime Sciences (TekMar), Vestfold University College (HiVE), Tønsberg, N3103, Norway
* Author to whom correspondence should be addressed.
Received: 10 September 2013; in revised form: 15 October 2013 / Accepted: 21 October 2013 / Published: 28 October 2013
Abstract: Mood disorders are common mental diseases, but physiological diagnostic methods are still lacking. Since much evidence has implied a relationship between mood disorders and the protein composition of blood sera, it is conceivable to develop a serological criterion for assisting diagnosis of mood disorders, based on a correlative database with enough capacity and high quality. In this pilot study, a low-cost microfluidic microarray device for quantifying at most 384 serological biomarkers at the same time was designed for the data acquisition of the serological study. The 1,536-chamber microfluidic device was modeled on a 1,536-well microtiter plate in order to employ a common microplate reader as the detection module for measuring the chemiluminescent immunoassay tests on the chips. The microfluidic microarrays were rapidly fabricated on polymethylmethacrylate slides using carbon dioxide laser ablation, followed by effective surface treatment processing. Sixteen types of different capture antibodies were immobilized on the chips to test the corresponding hormones and cytokines. The preliminary tests indicated that the signal-to-noise ratio and the limit of detection of microfluidic microarrays have reached the level of standard ELISA tests, whereas the operation time of microfluidic microarrays was sharply reduced.
Keywords: microfluidic microarray; lab on a chip; chemiluminescent immunoassay; carbon dioxide laser ablation; mood disorder
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MDPI and ACS Style
Zhao, X.; Dong, T. Design and Fabrication of Low-Cost 1536-Chamber Microfluidic Microarrays for Mood-Disorders-Related Serological Studies. Sensors 2013, 13, 14570-14582.
Zhao X, Dong T. Design and Fabrication of Low-Cost 1536-Chamber Microfluidic Microarrays for Mood-Disorders-Related Serological Studies. Sensors. 2013; 13(11):14570-14582.
Zhao, Xinyan; Dong, Tao. 2013. "Design and Fabrication of Low-Cost 1536-Chamber Microfluidic Microarrays for Mood-Disorders-Related Serological Studies." Sensors 13, no. 11: 14570-14582.