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Sensors 2013, 13(10), 14189-14199; doi:10.3390/s131014189
Article

Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves

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Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
* Author to whom correspondence should be addressed.
Received: 19 August 2013 / Revised: 26 September 2013 / Accepted: 30 September 2013 / Published: 22 October 2013
(This article belongs to the Section Biosensors)
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Abstract

Bacterial communication or quorum sensing (QS) is achieved via sensing of QS signaling molecules consisting of oligopeptides in Gram-positive bacteria and N-acyl homoserine lactones (AHL) in most Gram-negative bacteria. In this study, Enterobacteriaceae isolates from Batavia lettuce were screened for AHL production. Enterobacter asburiae, identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was found to produce short chain AHLs. High resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) analysis of the E. asburiae spent supernatant confirmed the production of N-butanoyl homoserine lactone (C4-HSL) and N–hexanoyl homoserine lactone (C6-HSL). To the best of our knowledge, this is the first report of AHL production by E. asburiae.
Keywords: Enterobacter asburiae; MALDI-TOF; mass spectrometry; N-acyl homoserine lactone; N–hexanoyl homoserine lactone; N-butanoyl homoserine lactone; quorum sensing; lettuce; food spoilage; food microbiology; food safety Enterobacter asburiae; MALDI-TOF; mass spectrometry; N-acyl homoserine lactone; N–hexanoyl homoserine lactone; N-butanoyl homoserine lactone; quorum sensing; lettuce; food spoilage; food microbiology; food safety
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Lau, Y.Y.; Sulaiman, J.; Chen, J.W.; Yin, W.-F.; Chan, K.-G. Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves. Sensors 2013, 13, 14189-14199.

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