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Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
* Author to whom correspondence should be addressed.
Received: 19 August 2013; in revised form: 26 September 2013 / Accepted: 30 September 2013 / Published: 22 October 2013
Abstract: Bacterial communication or quorum sensing (QS) is achieved via sensing of QS signaling molecules consisting of oligopeptides in Gram-positive bacteria and N-acyl homoserine lactones (AHL) in most Gram-negative bacteria. In this study, Enterobacteriaceae isolates from Batavia lettuce were screened for AHL production. Enterobacter asburiae, identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was found to produce short chain AHLs. High resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) analysis of the E. asburiae spent supernatant confirmed the production of N-butanoyl homoserine lactone (C4-HSL) and N–hexanoyl homoserine lactone (C6-HSL). To the best of our knowledge, this is the first report of AHL production by E. asburiae.
Keywords: Enterobacter asburiae; MALDI-TOF; mass spectrometry; N-acyl homoserine lactone; N–hexanoyl homoserine lactone; N-butanoyl homoserine lactone; quorum sensing; lettuce; food spoilage; food microbiology; food safety
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MDPI and ACS Style
Lau, Y.Y.; Sulaiman, J.; Chen, J.W.; Yin, W.-F.; Chan, K.-G. Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves. Sensors 2013, 13, 14189-14199.
Lau YY, Sulaiman J, Chen JW, Yin W-F, Chan K-G. Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves. Sensors. 2013; 13(10):14189-14199.
Lau, Yin Y.; Sulaiman, Joanita; Chen, Jian W.; Yin, Wai-Fong; Chan, Kok-Gan. 2013. "Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves." Sensors 13, no. 10: 14189-14199.