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Sensors 2013, 13(10), 14189-14199; doi:10.3390/s131014189
Article
Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
* Author to whom correspondence should be addressed.
Received: 19 August 2013; in revised form: 26 September 2013 / Accepted: 30 September 2013 / Published: 22 October 2013
(This article belongs to the Section Biosensors)
Download PDF Full-Text [323 KB, Updated Version, uploaded 23 October 2013 14:37 CEST]
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Abstract: Bacterial communication or quorum sensing (QS) is achieved via sensing of QS signaling molecules consisting of oligopeptides in Gram-positive bacteria and N-acyl homoserine lactones (AHL) in most Gram-negative bacteria. In this study, Enterobacteriaceae isolates from Batavia lettuce were screened for AHL production. Enterobacter asburiae, identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was found to produce short chain AHLs. High resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) analysis of the E. asburiae spent supernatant confirmed the production of N-butanoyl homoserine lactone (C4-HSL) and N–hexanoyl homoserine lactone (C6-HSL). To the best of our knowledge, this is the first report of AHL production by E. asburiae.
Keywords: Enterobacter asburiae; MALDI-TOF; mass spectrometry; N-acyl homoserine lactone; N–hexanoyl homoserine lactone; N-butanoyl homoserine lactone; quorum sensing; lettuce; food spoilage; food microbiology; food safety
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MDPI and ACS Style
Lau, Y.Y.; Sulaiman, J.; Chen, J.W.; Yin, W.-F.; Chan, K.-G. Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves. Sensors 2013, 13, 14189-14199.
AMA StyleLau YY, Sulaiman J, Chen JW, Yin W-F, Chan K-G. Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves. Sensors. 2013; 13(10):14189-14199.
Chicago/Turabian StyleLau, Yin Y.; Sulaiman, Joanita; Chen, Jian W.; Yin, Wai-Fong; Chan, Kok-Gan. 2013. "Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves." Sensors 13, no. 10: 14189-14199.