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Sensors 2013, 13(10), 14189-14199; doi:10.3390/s131014189
Article

Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves

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Received: 19 August 2013; in revised form: 26 September 2013 / Accepted: 30 September 2013 / Published: 22 October 2013
(This article belongs to the Section Biosensors)
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Abstract: Bacterial communication or quorum sensing (QS) is achieved via sensing of QS signaling molecules consisting of oligopeptides in Gram-positive bacteria and N-acyl homoserine lactones (AHL) in most Gram-negative bacteria. In this study, Enterobacteriaceae isolates from Batavia lettuce were screened for AHL production. Enterobacter asburiae, identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was found to produce short chain AHLs. High resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) analysis of the E. asburiae spent supernatant confirmed the production of N-butanoyl homoserine lactone (C4-HSL) and N–hexanoyl homoserine lactone (C6-HSL). To the best of our knowledge, this is the first report of AHL production by E. asburiae.
Keywords: Enterobacter asburiae; MALDI-TOF; mass spectrometry; N-acyl homoserine lactone; N–hexanoyl homoserine lactone; N-butanoyl homoserine lactone; quorum sensing; lettuce; food spoilage; food microbiology; food safety Enterobacter asburiae; MALDI-TOF; mass spectrometry; N-acyl homoserine lactone; N–hexanoyl homoserine lactone; N-butanoyl homoserine lactone; quorum sensing; lettuce; food spoilage; food microbiology; food safety
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lau, Y.Y.; Sulaiman, J.; Chen, J.W.; Yin, W.-F.; Chan, K.-G. Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves. Sensors 2013, 13, 14189-14199.

AMA Style

Lau YY, Sulaiman J, Chen JW, Yin W-F, Chan K-G. Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves. Sensors. 2013; 13(10):14189-14199.

Chicago/Turabian Style

Lau, Yin Y.; Sulaiman, Joanita; Chen, Jian W.; Yin, Wai-Fong; Chan, Kok-Gan. 2013. "Quorum Sensing Activity of Enterobacter asburiae Isolated from Lettuce Leaves." Sensors 13, no. 10: 14189-14199.



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