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Nucleic Acids for Ultra-Sensitive Protein Detection
MeBioS Biosensor Group, Faculteit Bio-Ingenieurswetenschappen, KU Leuven, Willem De Croylaan, Leuven, Belgium
* Author to whom correspondence should be addressed.
Received: 21 November 2012; in revised form: 26 December 2012 / Accepted: 28 December 2012 / Published: 21 January 2013
Abstract: Major advancements in molecular biology and clinical diagnostics cannot be brought about strictly through the use of genomics based methods. Improved methods for protein detection and proteomic screening are an absolute necessity to complement to wealth of information offered by novel, high-throughput sequencing technologies. Only then will it be possible to advance insights into clinical processes and to characterize the importance of specific protein biomarkers for disease detection or the realization of “personalized medicine”. Currently however, large-scale proteomic information is still not as easily obtained as its genomic counterpart, mainly because traditional antibody-based technologies struggle to meet the stringent sensitivity and throughput requirements that are required whereas mass-spectrometry based methods might be burdened by significant costs involved. However, recent years have seen the development of new biodetection strategies linking nucleic acids with existing antibody technology or replacing antibodies with oligonucleotide recognition elements altogether. These advancements have unlocked many new strategies to lower detection limits and dramatically increase throughput of protein detection assays. In this review, an overview of these new strategies will be given.
Keywords: nucleic acid; biosensor; protein; biomarker; PCR; aptamer
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Janssen, K.P.F.; Knez, K.; Spasic, D.; Lammertyn, J. Nucleic Acids for Ultra-Sensitive Protein Detection. Sensors 2013, 13, 1353-1384.
Janssen KPF, Knez K, Spasic D, Lammertyn J. Nucleic Acids for Ultra-Sensitive Protein Detection. Sensors. 2013; 13(1):1353-1384.
Janssen, Kris P.F.; Knez, Karel; Spasic, Dragana; Lammertyn, Jeroen. 2013. "Nucleic Acids for Ultra-Sensitive Protein Detection." Sensors 13, no. 1: 1353-1384.