Next Article in Journal
Accuracy and Precision of Equine Gait Event Detection during Walking with Limb and Trunk Mounted Inertial Sensors
Previous Article in Journal
Process-in-Network: A Comprehensive Network Processing Approach
Previous Article in Special Issue
Electrical Characterization of Gold-DNA-Gold Structures in Presence of an External Magnetic Field by Means of I-V Curve Analysis
Article Menu

Export Article

Open AccessArticle
Sensors 2012, 12(6), 8135-8144; doi:10.3390/s120608135

Novel Platform Development Using an Assembly of Carbon Nanotube, Nanogold and Immobilized RNA Capture Element towards Rapid, Selective Sensing of Bacteria

Molecular Bioeffects Branch, Human Effectiveness Directorate AFRL/RHDJ, Wright Patterson Air Force Base, Dayton, OH 45433, USA
Center for Nanoscale Multifunctional Materials, Wright State University, Dayton, OH 45435, USA
Author to whom correspondence should be addressed.
Received: 1 May 2012 / Revised: 29 May 2012 / Accepted: 1 June 2012 / Published: 12 June 2012
(This article belongs to the Special Issue Nano-Biosensors)
View Full-Text   |   Download PDF [871 KB, uploaded 21 June 2014]   |  


This study examines the creation of a nano-featured biosensor platform designed for the rapid and selective detection of the bacterium Escherichia coli. The foundation of this sensor is carbon nanotubes decorated with gold nanoparticles that are modified with a specific, surface adherent ribonucleiuc acid (RNA) sequence element. The multi-step sensor assembly was accomplished by growing carbon nanotubes on a graphite substrate, the direct synthesis of gold nanoparticles on the nanotube surface, and the attachment of thiolated RNA to the bound nanoparticles. The application of the compounded nano-materials for sensor development has the distinct advantage of retaining the electrical behavior property of carbon nanotubes and, through the gold nanoparticles, incorporating an increased surface area for additional analyte attachment sites, thus increasing sensitivity. We successfully demonstrated that the coating of gold nanoparticles with a selective RNA sequence increased the capture of E. coli by 189% when compared to uncoated particles. The approach to sensor formation detailed in this study illustrates the great potential of unique composite structures in the development of a multi-array, electrochemical sensor for the fast and sensitive detection of pathogens. View Full-Text
Keywords: biosensor; gold nanoparticle; carbon nanotube; nanomaterial; aptamer biosensor; gold nanoparticle; carbon nanotube; nanomaterial; aptamer

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Maurer, E.I.; Comfort, K.K.; Hussain, S.M.; Schlager, J.J.; Mukhopadhyay, S.M. Novel Platform Development Using an Assembly of Carbon Nanotube, Nanogold and Immobilized RNA Capture Element towards Rapid, Selective Sensing of Bacteria. Sensors 2012, 12, 8135-8144.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top