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Acetylcholinesterase Immobilized on Magnetic Beads for Pesticides Detection: Application to Olive Oil Analysis
IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France
AQUAMAR Laboratory, Photocatalysis and Environment Team, Department of Chemistry, Faculty of Science, University Ibn Zohr, BP 8106 Cité Dakhla, Agadir, Morocco
* Author to whom correspondence should be addressed.
Received: 4 May 2012; in revised form: 24 May 2012 / Accepted: 4 June 2012 / Published: 8 June 2012
Abstract: This work presents the development of bioassays and biosensors for the detection of insecticides widely used in the treatment of olive trees. The systems are based on the covalent immobilisation of acetylcholinesterase on magnetic microbeads using either colorimetry or amperometry as detection technique. The magnetic beads were immobilised on screen-printed electrodes or microtitration plates and tested using standard solutions and real samples. The developed devices showed good analytical performances with limits of detection much lower than the maximum residue limit tolerated by international regulations, as well as a good reproducibility and stability.
Keywords: electric eel acetylcholinesterase; organophosphorus insecticides; magnetic microbeads; olive oil
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MDPI and ACS Style
Ben Oujji, N.; Bakas, I.; Istamboulié, G.; Ait-Ichou, I.; Ait-Addi, E.; Rouillon, R.; Noguer, T. Acetylcholinesterase Immobilized on Magnetic Beads for Pesticides Detection: Application to Olive Oil Analysis. Sensors 2012, 12, 7893-7904.
Ben Oujji N, Bakas I, Istamboulié G, Ait-Ichou I, Ait-Addi E, Rouillon R, Noguer T. Acetylcholinesterase Immobilized on Magnetic Beads for Pesticides Detection: Application to Olive Oil Analysis. Sensors. 2012; 12(6):7893-7904.
Ben Oujji, Najwa; Bakas, Idriss; Istamboulié, Georges; Ait-Ichou, Ihya; Ait-Addi, Elhabib; Rouillon, Régis; Noguer, Thierry. 2012. "Acetylcholinesterase Immobilized on Magnetic Beads for Pesticides Detection: Application to Olive Oil Analysis." Sensors 12, no. 6: 7893-7904.