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Sensors 2012, 12(3), 3762-3772; doi:10.3390/s120303762
Article

A Pro-Drug Approach for Selective Modulation of AI-2-Mediated Bacterial Cell-to-Cell Communication

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Received: 29 December 2011; in revised form: 26 February 2012 / Accepted: 15 March 2012 / Published: 21 March 2012
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Abstract: The universal quorum sensing autoinducer, AI-2, is utilized by several bacteria. Analogs of AI-2 have the potential to modulate bacterial behavior. Selectively quenching the communication of a few bacteria, in the presence of several others in an ecosystem, using analogs of AI-2 is non-trivial due to the ubiquity of AI-2 processing receptors in many bacteria that co-exist. Herein, we demonstrate that when an AI-2 analog, isobutyl DPD (which has been previously shown to be a quorum sensing, QS, quencher in both Escherichia coli and Salmonella typhimurium) is modified with ester groups, which get hydrolyzed once inside the bacterial cells, only QS in E. coli, but not in S. typhimurium, is inhibited. The origin of this differential QS inhibition could be due to differences in analog permeation of the bacterial membranes or ester hydrolysis rates. Such differences could be utilized to selectively target QS in specific bacteria amongst a consortium of other species that also use AI-2 signaling.
Keywords: AI-2; DPD analog; quorum sensing inhibitor; ester pro-drug; lsr expression; autoinducer cell permeation; autoinducer processing; enteric bacteria AI-2; DPD analog; quorum sensing inhibitor; ester pro-drug; lsr expression; autoinducer cell permeation; autoinducer processing; enteric bacteria
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Guo, M.; Gamby, S.; Nakayama, S.; Smith, J.; Sintim, H.O. A Pro-Drug Approach for Selective Modulation of AI-2-Mediated Bacterial Cell-to-Cell Communication. Sensors 2012, 12, 3762-3772.

AMA Style

Guo M, Gamby S, Nakayama S, Smith J, Sintim HO. A Pro-Drug Approach for Selective Modulation of AI-2-Mediated Bacterial Cell-to-Cell Communication. Sensors. 2012; 12(3):3762-3772.

Chicago/Turabian Style

Guo, Min; Gamby, Sonja; Nakayama, Shizuka; Smith, Jacqueline; Sintim, Herman O. 2012. "A Pro-Drug Approach for Selective Modulation of AI-2-Mediated Bacterial Cell-to-Cell Communication." Sensors 12, no. 3: 3762-3772.



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