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N-Acyl Homoserine Lactones in Diverse Pectobacterium and Dickeya Plant Pathogens: Diversity, Abundance, and Involvement in Virulence
Laboratoire de Microbiologie Signaux et Microenvironnement, Normandie Université, Université de Rouen EA 4312, IUT Evreux, 55 rue Saint-Germain, Evreux F-27000, France
SIPRE—Comité Nord, Station de recherche et de création variétale, Bretteville du Grand Caux F-76110, France
Institut des Sciences du Végétal, CNRS UPR2355, Avenue de la Terrasse, Gif-sur-Yvette 91198, France
FN3PT, Fédération Nationale des Producteurs de Plants de Pomme de Terre, 43-45 rue de Naples, Paris F-75008, France
INRA, UMR 1349 IGEPP, Le Rheu Cedex F-35653, France
* Author to whom correspondence should be addressed.
Received: 15 February 2012; in revised form: 2 March 2012 / Accepted: 6 March 2012 / Published: 12 March 2012
Abstract: Soft-rot bacteria Pectobacterium and Dickeya use N-acyl homoserine lactones (NAHSLs) as diffusible signals for coordinating quorum sensing communication. The production of NAHSLs was investigated in a set of reference strains and recently-collected isolates, which belong to six species and share the ability to infect the potato host plant. All the pathogens produced different NAHSLs, among which the 3-oxo-hexanoyl- and the 3-oxo-octanoyl-L-homoserine lactones represent at least 90% of total produced NAHSL-amounts. The level of NAHSLs varied from 0.6 to 2 pg/cfu. The involvement of NAHSLs in tuber maceration was investigated by electroporating a quorum quenching vector in each of the bacterial pathogen strains. All the NAHSL-lactonase expressing strains produced a lower amount of NAHSLs as compared to those harboring the empty vector. Moreover, all except Dickeya dadantii 3937 induced a lower level of symptoms in potato tuber assay. Noticeably, aggressiveness appeared to be independent of both nature and amount of produced signals. This work highlights that quorum sensing similarly contributed to virulence in most of the tested Pectobacterium and Dickeya, even the strains had been isolated recently or during the past decades. Thus, these key regulatory-molecules appear as credible targets for developing anti-virulence strategies against these plant pathogens.
Keywords: soft-rot bacteria; Solanum tuberosum L.; N-acyl homoserine lactones; quorum sensing; AttM lactonase; quorum quenching
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Crépin, A.; Beury-Cirou, A.; Barbey, C.; Farmer, C.; Hélias, V.; Burini, J.-F.; Faure, D.; Latour, X. N-Acyl Homoserine Lactones in Diverse Pectobacterium and Dickeya Plant Pathogens: Diversity, Abundance, and Involvement in Virulence. Sensors 2012, 12, 3484-3497.
Crépin A, Beury-Cirou A, Barbey C, Farmer C, Hélias V, Burini J-F, Faure D, Latour X. N-Acyl Homoserine Lactones in Diverse Pectobacterium and Dickeya Plant Pathogens: Diversity, Abundance, and Involvement in Virulence. Sensors. 2012; 12(3):3484-3497.
Crépin, Alexandre; Beury-Cirou, Amélie; Barbey, Corinne; Farmer, Christine; Hélias, Valérie; Burini, Jean-François; Faure, Denis; Latour, Xavier. 2012. "N-Acyl Homoserine Lactones in Diverse Pectobacterium and Dickeya Plant Pathogens: Diversity, Abundance, and Involvement in Virulence." Sensors 12, no. 3: 3484-3497.