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Sensors 2012, 12(12), 16879-16891; doi:10.3390/s121216879
Article

Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels

1,* , 2,* , 2
,
2
 and
1
Received: 29 October 2012 / Revised: 30 November 2012 / Accepted: 4 December 2012 / Published: 6 December 2012
(This article belongs to the Special Issue Live Cell-Based Sensors)
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Abstract

A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 µM, obtained with the strain C. reinhardtii after 40 minutes of exposure.
Keywords: biosensor; algae; chlorophyll fluorescence; DCMU; atrazine; sol-gel; herbicides biosensor; algae; chlorophyll fluorescence; DCMU; atrazine; sol-gel; herbicides
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ferro, Y.; Perullini, M.; Jobbagy, M.; Bilmes, S.A.; Durrieu, C. Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels. Sensors 2012, 12, 16879-16891.

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