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Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
Université de Lyon, ENTPE, CNRS, UMR5023 LEHNA, Rue Maurice Audin, 69518 Vaulx-en-Velin, France
INQUIMAE-DQIAQF, Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria Pab. II, C1428EHA Buenos Aires, Argentina
* Authors to whom correspondence should be addressed.
Received: 29 October 2012; in revised form: 30 November 2012 / Accepted: 4 December 2012 / Published: 6 December 2012
Abstract: A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 µM, obtained with the strain C. reinhardtii after 40 minutes of exposure.
Keywords: biosensor; algae; chlorophyll fluorescence; DCMU; atrazine; sol-gel; herbicides
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Ferro, Y.; Perullini, M.; Jobbagy, M.; Bilmes, S.A.; Durrieu, C. Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels. Sensors 2012, 12, 16879-16891.
Ferro Y, Perullini M, Jobbagy M, Bilmes SA, Durrieu C. Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels. Sensors. 2012; 12(12):16879-16891.
Ferro, Yannis; Perullini, Mercedes; Jobbagy, Matias; Bilmes, Sara A.; Durrieu, Claude. 2012. "Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels." Sensors 12, no. 12: 16879-16891.