Next Article in Journal
Evaluation of the Effectiveness of Five Odor Reducing Agents for Sewer System Odors Using an On-Line Total Reduced Sulfur Analyzer
Next Article in Special Issue
Synthetic and Bio-Artificial Tactile Sensing: A Review
Previous Article in Journal
Investigation into the Effect of Varied Functional Biointerfaces on Silicon Nanowire MOSFETs
Previous Article in Special Issue
Design, Fabrication and Characterization of a Low-Impedance 3D Electrode Array System for Neuro-Electrophysiology
Article Menu

Export Article

Open AccessArticle
Sensors 2012, 12(12), 16879-16891; doi:10.3390/s121216879

Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels

Université de Lyon, ENTPE, CNRS, UMR5023 LEHNA, Rue Maurice Audin, 69518 Vaulx-en-Velin, France
INQUIMAE-DQIAQF, Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria Pab. II, C1428EHA Buenos Aires, Argentina
Authors to whom correspondence should be addressed.
Received: 29 October 2012 / Revised: 30 November 2012 / Accepted: 4 December 2012 / Published: 6 December 2012
(This article belongs to the Special Issue Live Cell-Based Sensors)
View Full-Text   |   Download PDF [2104 KB, 21 June 2014; original version 21 June 2014]   |  


A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 µM, obtained with the strain C. reinhardtii after 40 minutes of exposure.
Keywords: biosensor; algae; chlorophyll fluorescence; DCMU; atrazine; sol-gel; herbicides biosensor; algae; chlorophyll fluorescence; DCMU; atrazine; sol-gel; herbicides

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ferro, Y.; Perullini, M.; Jobbagy, M.; Bilmes, S.A.; Durrieu, C. Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels. Sensors 2012, 12, 16879-16891.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top