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Sensors 2012, 12(12), 16614-16627; doi:10.3390/s121216614
Review

Development of a Novel, Fully-Automated Genotyping System: Principle and Applications

1
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 and 2,*
Received: 15 May 2012; in revised form: 2 October 2012 / Accepted: 26 November 2012 / Published: 3 December 2012
(This article belongs to the Special Issue Micro and Nano Technologies for Point-of-Care Diagnosis)
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Abstract: Genetic testing prior to treatment, pharmacogenetic analysis, is key to realizing personalized medicine which is a topic that has attracted much attention recently. Through the optimization of therapy selection and dosage, a reduction in side effects is expected. Genetic testing has been conducted as a type of pharmacogenetic analysis in recent years, but it faces challenges in terms of cost effectiveness and its complicated procedures. Here we report on the development of a novel platform for genetic testing, the i-densyTM, with the use of quenching probe system (QP-system) as principle of mutant detection. The i-densyTM automatically performs pre-treatment, PCR and detection to provide the test result from whole blood and extracted DNA within approximately 90 and 60 min, respectively. Integration of all steps into a single platform greatly reduces test time and complicated procedures. An even higher-precision genetic analysis has been achieved through the development of novel and highly-specific detection methods. The applications of items measured using the i-densyTM are diverse, from single nucleotide polymorphism (SNP), such as CYP2C19 and UGT1A1, to somatic mutations associated with cancer, such as EGFR, KRAS and JAK2. The i-densyTM is a useful tool for optimization of anticancer drug therapy and can contribute to personalized medicine.
Keywords: automated genotyping system; pharmacogenetic analysis; single nucleotide polymorphism; i-densyTM; Qprobe; mutation biased PCR; personalized medicine automated genotyping system; pharmacogenetic analysis; single nucleotide polymorphism; i-densyTM; Qprobe; mutation biased PCR; personalized medicine
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Suzuki, S.-I.; Komori, M.; Hirai, M.; Ureshino, N.; Kimura, S. Development of a Novel, Fully-Automated Genotyping System: Principle and Applications. Sensors 2012, 12, 16614-16627.

AMA Style

Suzuki S-I, Komori M, Hirai M, Ureshino N, Kimura S. Development of a Novel, Fully-Automated Genotyping System: Principle and Applications. Sensors. 2012; 12(12):16614-16627.

Chicago/Turabian Style

Suzuki, Shun-ichi; Komori, Mariko; Hirai, Mitsuharu; Ureshino, Norio; Kimura, Shinya. 2012. "Development of a Novel, Fully-Automated Genotyping System: Principle and Applications." Sensors 12, no. 12: 16614-16627.


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