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Development of a Novel, Fully-Automated Genotyping System: Principle and Applications
ARKRAY Inc., Kyoto 602-0008, Japan
Division of Haematology, Respiratory Medicine, and Oncology, Faculty of Medicine, Saga University, Saga 849-8501, Japan
Saga Prefectural Hospital Koseikan, Saga 840-8571, Japan
* Author to whom correspondence should be addressed.
Received: 15 May 2012; in revised form: 2 October 2012 / Accepted: 26 November 2012 / Published: 3 December 2012
Abstract: Genetic testing prior to treatment, pharmacogenetic analysis, is key to realizing personalized medicine which is a topic that has attracted much attention recently. Through the optimization of therapy selection and dosage, a reduction in side effects is expected. Genetic testing has been conducted as a type of pharmacogenetic analysis in recent years, but it faces challenges in terms of cost effectiveness and its complicated procedures. Here we report on the development of a novel platform for genetic testing, the i-densyTM, with the use of quenching probe system (QP-system) as principle of mutant detection. The i-densyTM automatically performs pre-treatment, PCR and detection to provide the test result from whole blood and extracted DNA within approximately 90 and 60 min, respectively. Integration of all steps into a single platform greatly reduces test time and complicated procedures. An even higher-precision genetic analysis has been achieved through the development of novel and highly-specific detection methods. The applications of items measured using the i-densyTM are diverse, from single nucleotide polymorphism (SNP), such as CYP2C19 and UGT1A1, to somatic mutations associated with cancer, such as EGFR, KRAS and JAK2. The i-densyTM is a useful tool for optimization of anticancer drug therapy and can contribute to personalized medicine.
Keywords: automated genotyping system; pharmacogenetic analysis; single nucleotide polymorphism; i-densyTM; Qprobe; mutation biased PCR; personalized medicine
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Suzuki, S.-I.; Komori, M.; Hirai, M.; Ureshino, N.; Kimura, S. Development of a Novel, Fully-Automated Genotyping System: Principle and Applications. Sensors 2012, 12, 16614-16627.
Suzuki S-I, Komori M, Hirai M, Ureshino N, Kimura S. Development of a Novel, Fully-Automated Genotyping System: Principle and Applications. Sensors. 2012; 12(12):16614-16627.
Suzuki, Shun-ichi; Komori, Mariko; Hirai, Mitsuharu; Ureshino, Norio; Kimura, Shinya. 2012. "Development of a Novel, Fully-Automated Genotyping System: Principle and Applications." Sensors 12, no. 12: 16614-16627.