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Sensors 2012, 12(11), 15281-15291; doi:10.3390/s121115281
Article

Simultaneous Measurement of Neural Spike Recordings and Multi-Photon Calcium Imaging in Neuroblastoma Cells

1
,
1
,
1
,
2,*  and 2,*
1 School of Electrical Engineering and Computer Science, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu 702-701, Korea 2 School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu 702-701, Korea
* Authors to whom correspondence should be addressed.
Received: 9 August 2012 / Revised: 5 November 2012 / Accepted: 6 November 2012 / Published: 8 November 2012
(This article belongs to the Special Issue Medical & Biological Imaging)
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Abstract

This paper proposes the design and implementation of a micro-electrode array (MEA) for neuroblastoma cell culturing. It also explains the implementation of a multi-photon microscope (MPM) customized for neuroblastoma cell excitation and imaging under ambient light. Electrical signal and fluorescence images were simultaneously acquired from the neuroblastoma cells on the MEA. MPM calcium images of the cultured neuroblastoma cell on the MEA are presented and also the neural activity was acquired through the MEA recording. A calcium green-1 (CG-1) dextran conjugate of 10,000 D molecular weight was used in this experiment for calcium imaging. This study also evaluated the calcium oscillations and neural spike recording of neuroblastoma cells in an epileptic condition. Based on our observation of neural spikes in neuroblastoma cells with our proposed imaging modality, we report that neuroblastoma cells can be an important model for epileptic activity studies.
Keywords: micro-electrode array; neural spike recording; multi-photon microscope micro-electrode array; neural spike recording; multi-photon microscope
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kim, S.; Jung, U.; Baek, J.; Kang, S.; Kim, J. Simultaneous Measurement of Neural Spike Recordings and Multi-Photon Calcium Imaging in Neuroblastoma Cells. Sensors 2012, 12, 15281-15291.

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