Next Article in Journal
Providing Self-Healing Ability for Wireless Sensor Node by Using Reconfigurable Hardware
Next Article in Special Issue
Indirect Determination of Mercury Ion by Inhibition of a Glucose Biosensor Based on ZnO Nanorods
Previous Article in Journal
A Robust Static Decoupling Algorithm for 3-Axis Force Sensors Based on Coupling Error Model and ε-SVR
Sensors 2012, 12(11), 14556-14569; doi:10.3390/s121114556

Catalytic and Inhibitory Kinetic Behavior of Horseradish Peroxidase on the Electrode Surface

* ,  and
Department of Chemistry and State Key Laboratory of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China
* Author to whom correspondence should be addressed.
Received: 2 August 2012 / Revised: 29 August 2012 / Accepted: 17 September 2012 / Published: 29 October 2012
(This article belongs to the Special Issue Enzymatic Biosensors)
View Full-Text   |   Download PDF [480 KB, uploaded 21 June 2014]   |   Browse Figures


Enzymatic biosensors are often used to detect trace levels of some specific substance. An alternative methodology is applied for enzymatic assays, in which the electrocatalytic kinetic behavior of enzymes is monitored by measuring the faradaic current for a variety of substrate and inhibitor concentrations. Here we examine a steady-state and pre-steady-state reduction of H2O2 on the horseradish peroxidase electrode. The results indicate the substrate-concentration dependence of the steady-state current strictly obeys Michaelis-Menten kinetics rules; in other cases there is ambiguity, whereby he inhibitor-concentration dependence of the steady-state current has a discontinuity under moderate concentration conditions. For pre-steady-state phases, both catalysis and inhibition show an abrupt change of the output current. These anomalous phenomena are universal and there might be an underlying biochemical or electrochemical rationale.
Keywords: enzymatic kinetics; inhibition; anomalous behavior; electrochemical biosensor enzymatic kinetics; inhibition; anomalous behavior; electrochemical biosensor
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Huang, J.; Huang, W.; Wang, T. Catalytic and Inhibitory Kinetic Behavior of Horseradish Peroxidase on the Electrode Surface. Sensors 2012, 12, 14556-14569.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert