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Sensors 2011, 11(6), 5740-5753; doi:10.3390/s110605740

Effects of Al(III) and Nano-Al13 Species on Malate Dehydrogenase Activity

1
Jiangsu Key Laboratory of Biofunctional Materials, Laboratory of Electrochemistry, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097, China
2
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
*
Author to whom correspondence should be addressed.
Received: 25 March 2011 / Revised: 12 May 2011 / Accepted: 20 May 2011 / Published: 27 May 2011
(This article belongs to the Section Chemical Sensors)
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Abstract

The effects of different aluminum species on malate dehydrogenase (MDH) activity were investigated by monitoring amperometric i-t curves for the oxidation of NADH at low overpotential using a functionalized multi-wall nanotube (MWNT) modified glass carbon electrode (GCE). The results showed that Al(III) and Al13 can activate the enzymatic activity of MDH, and the activation reaches maximum levels as the Al(III) and Al13 concentration increase. Our study also found that the effects of Al(III) and Al13 on the activity of MDH depended on the pH value and aluminum speciation. Electrochemical and circular dichroism spectra methods were applied to study the effects of nano-sized aluminum compounds on biomolecules. View Full-Text
Keywords: electroanalysis; spectroscopy; Al(III); Al13; malate dehydrogenase electroanalysis; spectroscopy; Al(III); Al13; malate dehydrogenase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Yang, X.; Cai, L.; Peng, Y.; Li, H.; Chen, R.F.; Shen, R.F. Effects of Al(III) and Nano-Al13 Species on Malate Dehydrogenase Activity. Sensors 2011, 11, 5740-5753.

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