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Development of a Stepping Force Analgesic Meter for a Rat Arthritic Model
School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia
Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Malaysia
Faculty of Computer Science and Information Technology, University of Malaya, 50603, Kuala Lumpur, Malaysia
Malaysian Institute of Information Technology, Universiti Kuala Lumpur, 50250, Kuala Lumpur, Malaysia
* Author to whom correspondence should be addressed.
Received: 7 February 2011; in revised form: 19 April 2011 / Accepted: 29 April 2011 / Published: 5 May 2011
Abstract: Behavioural assessment of experimental pain is an essential method for analysing and measuring pain levels. Rodent models, which are widely used in behavioural tests, are often subject to external forces and stressful manipulations that cause variability of the parameters measured during the experiment. Therefore, these parameters may be inappropriate as indicators of pain. In this article, a stepping-force analgesimeter was designed to investigate the variations in the stepping force of rats in response to pain induction. The proposed apparatus incorporates new features, namely an infrared charge-coupled device (CCD) camera and a data acquisition system. The camera was able to capture the locomotion of the rats and synchronise the stepping force concurrently so that each step could be identified. Inter-day and intra-day precision and accuracy of each channel (there were a total of eight channels in the analgesimeter and each channel was connected to one load cell and one amplifier) were studied using different standard load weights. The validation studies for each channel also showed convincing results whereby intra-day and inter-day precision were less than 1% and accuracy was 99.36–100.36%. Consequently, an in vivo test was carried out using 16 rats (eight females and eight males). The rats were allowed to randomly walk across the sensor tunnel (the area that contained eight channels) and the stepping force and locomotion were recorded. A non-expert, but from a related research domain, was asked to differentiate the peaks of the front and hind paw, respectively. The results showed that of the total movement generated by the rats, 50.27 ± 3.90% in the case of the male rats and 62.20 ± 6.12% in that of the female rats had more than two peaks, a finding which does not substantiate the assumptions made in previous studies. This study also showed that there was a need to use the video display frame to distinguish between the front and hind paws in the case of 48.80 ± 4.01% of the male rats and 66.76 ± 5.35% of the female rats. Evidently the assumption held by current researchers regarding stepping force measurement is not realistic in terms of application, and as this study has shown, the use of a video display frame is essential for the identification of the front and hind paws through the peak signals.
Keywords: arthritis; analgesimeter; nociception; stepping force; load cells
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Yam, M.F.; Por, L.Y.; Peh, K.K.; Ahmad, M.; Asmawi, M.Z.; Ang, L.F.; Yin, D.B.M.; Ong, S.Y.; Abdulkarim, M.F.; Abdullah, G.Z.; Salman, I.M.; Ameer, O.Z.; Mohamed, E.A.H.; Hashim, M.A.; Farsi, E.; Hor, S.Y. Development of a Stepping Force Analgesic Meter for a Rat Arthritic Model. Sensors 2011, 11, 5058-5070.
Yam MF, Por LY, Peh KK, Ahmad M, Asmawi MZ, Ang LF, Yin DBM, Ong SY, Abdulkarim MF, Abdullah GZ, Salman IM, Ameer OZ, Mohamed EAH, Hashim MA, Farsi E, Hor SY. Development of a Stepping Force Analgesic Meter for a Rat Arthritic Model. Sensors. 2011; 11(5):5058-5070.
Yam, Mun Fei; Por, Lip Yee; Peh, Kok Khiang; Ahmad, Mariam; Asmawi, Mohd. Zaini; Ang, Lee Fung; Yin, Delina Beh Mei; Ong, Sim Ying; Abdulkarim, Muthanna Fawzy; Abdullah, Ghassan Zuhair; Salman, Ibrahim Muhammad; Ameer, Omar Ziad; Mohamed, Elsnoussi Ali Hussin; Hashim, Mohd Akmal; Farsi, Elham; Hor, Sook Yee. 2011. "Development of a Stepping Force Analgesic Meter for a Rat Arthritic Model." Sensors 11, no. 5: 5058-5070.